口服类肝素理化性质和药理活性的研究

目的：制备口服类肝素样品并研究其理化性质、促脂酶释放活性和抑制血栓形成作用。方法：猪十二指肠通过提取分离得到类肝素样品，采用常规方法测定其抗Xa因子活性、活化部分凝血激酶时间(APTT)活性等各项理化性质，采用比色测定法考察其促进肝脂酶(HL)和脂蛋白脂酶(LPL)释放活性，并采用大鼠动静脉旁路血栓模型考察其口服给药对血栓形成的抑制作用。结果：类肝素样品抗Xa因子活性70.4IU／mg，APTT活性33.8IU／mg，FXa／FIIa为2．1，促HL和LPL释放及抑制大鼠动静脉旁路血栓作用显著。结论：类肝素样品具有显著的促HL和LPL释放及口服抑制血栓形成作用。

Experimental Research of Physicochemical Properties and Pharmacological Action of a Peroral Heparinoid

To study the physicochemical properties,ant ilipaemic activity a nd anti thrombotic effect of a domestic porcine duodenum heparinoid. Th e heparinoid was obtained from porcine duodenum by extraction and puri fication. Its physicochemical properties such as anti Xa and APTT activity were studied and examined; the effects on activities of HL and LPL in plasma of rat s were studied by chromometry and the effect on thrombosis was examined on arter ove nous shunt model in rats. The anti Xa and APTT activities were 70 4 and 33 8 IU/mg, respectively. FXa/FIIa was 2 1 The heparinoid showed markedly the prom oting rel ease of HL and LPL activities in plasma of rats and opposing the in cidence of th rombosis on arterovenous shunt model of rats. The hepa rinoid has shown significantly antilipaemic effect and preventing thrombosis on animal thrombotic model.