不同方法检测乙肝表面抗原的结果分析

目的分析酶联免疫吸附法(ELISA)和电化学发光免疫法((ECLIA)检测乙肝表面抗原(HBsAg)的结果及临床应用价值。方法收集经ELISA法检测过的120份临床患者的血清,其中包括:30份HBsAg阳性;30份HBsAg阴性但乙肝e抗体(eAb)和乙肝核心抗体(cAb)阳性;30份HBsAg阴性但cAb阳性;30份乙肝5项均阴性。对上述血清采用ECLIA进行HBsAg检测,并对检测结果进行比较分析。结果 30份经ELISA检测HBsAg阳性者经ECLIA检测均为阳性,其灵敏度达100.0%;30份经ELISA检测HBsAg阴性而eAb、cAb阳性者中,有4份血清经ECLIA检测HBsAg呈阳性,其阳性率达13.3%;30份经ELISA检测HBsAg阴性而cAb阳性者中,有2份血清经ECLIA检测HBsAg呈阳性,其阳性率达6.7%;30份血清经ELISA检测乙肝5项均阴性者中,有2份血清经ECLIA检测HBsAg呈阳性,其阳性率达6.7%。结论 ECLIA法较ELISA法敏感性更高;对临床避免医院内有创检查、手术、输血等情况下的乙肝感染和传播等具有重要应用价值。

Analysis of the Results of Hepatitis B Surface Antigen Detected by Different Methods

Objective To analyze the results and the clinical application of HBsAg which was detected by the methods of ELISA and ECLIA. Methods To collect 120 copiesof patients＇ serum that detected by the method of ELISA . The serum include 30 copies of HBsAg positive serum, 30 copies of HBsAg negative but HBeAb and HBcAb positive serum, 30 copies of HBsAg negative but HBcAb positive serum and 30 copies of Hepatitis B five items detection were all negative serum. To detect the above sera by the method of ELICA, then compare and analyze the test results. Results 30 copies of HBsAg positive serum were all positive after detected by ECLIA ,and the sensitivity rate is 100.0%. In the 30 copies of HBsAg negative but HBeAb and HBcAb positive serum ,there were 4 copies positive after detected by ECLIA ,and the sensitivity rate was 13. 3%. In the 30 copies of HBsAg negative but HBcAb positive and 30 copies of Hepatitis B detection were all negative serum, there were 2 copies positive after detected by ECLIA, the sensitivity rate were both 6.7%. Conclusion The method of ECLIA had higher sensitivity and specificity. It can reflect the HBV infection status of the patients more accurately. It is more suitable for early diagnosis, treatment and curative effect assessment of HBV infection. It has important significance to avoid the infection and dissemination of HBV in the invasive examination, operation and blood transfusion.