| HPLC法测定人血浆中多沙普仑的浓度 |
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| 引用本文： | 程佳,夏东亚,郭涛,唐云彪,颜鸣,隋因.HPLC法测定人血浆中多沙普仑的浓度[J].中国药师,2010,13(5):618-619. |
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| 作者姓名： | 程佳 夏东亚 郭涛 唐云彪 颜鸣 隋因 |
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| 作者单位： | 1. 沈阳药科大学,沈阳,110016
2. 沈阳军区总医院药剂科 |
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| 基金项目： | 全军医学科学技术研究"十一五"计划科技攻关课题基金 |
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| 摘 要： | 目的：建立测定人血浆中多沙普仑浓度的HPLC法。方法：血浆样品经液-液萃取处理后进行测定。色谱柱为Dia—monsil C18柱（200mm×4．6mm，5μm）；柱温为室温；流动相为磷酸二氢铵缓冲液（0．01Mol·L^-1，pH4．5）．甲醇-乙腈（56：30：14）；流速为1．3ml·min^-1；紫外检测波长为214nm；内标为非那西丁。结果：多沙普仑血药浓度在0．05-5μg·ml^-1范围内线性关系良好（r=0．9990）；提取回收率为60．6％～64．0％；日内RSD小于6．5％，日间RSD小于13．4％。结论：此方法操作简便，灵敏，准确，适用于多沙普仑的临床药物动力学研究。
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| 关 键 词： | 多沙普仑 高效液相色谱法 血药浓度 |
Determination of Doxapram in Human Plasma by HPLC |
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| Cheng Jia,Xia Dongya,Guo Tao,Tang Yunbiao,Yan Ming,Sui Yin.Determination of Doxapram in Human Plasma by HPLC[J].China Pharmacist,2010,13(5):618-619. |
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| Authors: | Cheng Jia Xia Dongya Guo Tao Tang Yunbiao Yan Ming Sui Yin |
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| Institution: | Cheng Jia~1,Xia Dongya~2,Guo Tao~2,Tang Yunbiao~2,Yan Ming~2,Sui Yin~2(1.Shenyang Pharmaceutical University,Shenyang 110016,China,2.Department of Pharmacy,General Hospital of Shenyang Military Region) |
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| Abstract: | Objective ： To establish a RP-HPLC method for the determination of doxapram in human plasma. Method： The plasma sample was analyzed after liquid-liquid extraction. The Diamonsil C18 （ 200 mm ×4.6 mm ,5 μm） column was used with a mobile phase consisted of a mixture of 0.01 mol · L^-1 ammonium dihydrogen phosphate-methanol-acetonitrile（56： 30： 14）. The flow rate was 1.3 ml · min^-1. The UV detector was set at 214 nm. The internal standard method was used to quantify doxapram. Result： The linear calibration curve was obtained in the concentration range of 0.05 to 5 μg · ml^- 1 （ r = 0. 999 0）. The extraction recoveries at different levels were between 60.6% - 64.0%. The intra-day RSD were less than 6.5% and inter-day RSD were less than 13.4%. Conclusion： The method can be used for the pharmacokinetic study of doxapram. |
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| Keywords: | Doxapram HPLC Plasma concentration |
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