黄连素抑制慢性心衰大鼠心肌炎性反应

 目的 在慢性心衰(CHF)大鼠模型检测Toll样受体4(TLR4)的表达，探讨黄连素在慢性心衰炎性反应干预中可能的作用机制。方法 大鼠每周腹腔注射阿霉素2.8mg/kg共10周建立慢性心衰模型，将其随机分为黄连素组、安慰剂组及对照组，每组10只。黄连素组给予黄连素每天21mg/kg灌胃4周，另两组给予等量生理盐水。用酶联吸附免疫反应法(ELISA)检测大鼠血清中BNP、TNFα、IL-1及IL-6的含量；qRT-PCR法检测大鼠血清诱生型一氧化氮合酶(iNOS)表达；HE染色观察大鼠心肌细胞形态学改变；免疫组织化学观察大鼠心肌TLR4蛋白表达和分布。结果 黄连素组大鼠血清TNFα、IL-1、IL-6及iNOS mRNA水平显著低于安慰剂组(P<0.05)；黄连素组及安慰剂组TLR4吸光度值均高于对照组，而黄连素组显著低于安慰剂组(P<0.05)。结论 黄连素可能通过抑制TLR4 信号传导抑制慢性心衰炎性反应。

Berberine inhibits myocardial inflammation in rat with chronic heart failure

Objiective To detect the expression of Toll Like Receptors4 (TLR4) in experimental model rat with chronic heart failure (CHF), and investigate the possible mechanism of berberine treating inflammation response of CHF. Methods Rat model of CHF was established by intraperitoneal injection of adriamycin with 2.8 mg/kg per week for 10 weeks. The rat model of CHF were randomly divided into berberine group,placebo group and normal group.Each group contained 10 rats. Berberine group was given berberine with 21 mg/kg per day for 4 weeks while other groups were given distilled saline water. Serum BNP, TNF-α,IL-1and IL-6 were assessed by ELISA,iNOS mRNA was measured by qRT-PCR,TLR4 protein expression and distribution were detected by immunohistochemistry. HE stain was performed to observe morphological features. Results Serum BNP,TNF-α,IL-1,IL-6 and iNOS mRNA level in berberine group were significantly attenuated compared with in placebo group (P<0.05).TLR4 Absorbance value in berberine and placebo group were enhanced compared with normal group (P<0.05), while in berberine group significant decreased compared with in placebo group(P<0.05). Conclusion Berberine can inhibit the inflammatory response in CHF may through down-regulation signal transduction of TLR4．