云南省1997-2000年及2004年非脊髓灰质炎肠道病毒分子生物学及流行特征分析

目的描述云南省1997-2000年及2004年5年的肠道病毒（EV）分离状况及型别特征。方法5年在急性迟缓性麻痹病例中共检测到210株非脊髓灰质炎（脊灰）肠道病毒（NPEVs）,其中12株经血清学鉴定为腺病毒。剩余198株经基因测序定型,即病毒VP1区基因序列转变为氨基酸序列后与标准株进行比较。结果经分子生物学定型：人类EV-A组5株（5个血清型）、EV-B组158株（34个血清型）、EV-C组32株（6个血清型）、未分离到EV-D组病毒。在急性迟缓性麻痹病例监测中,210个分离株,EV-B组病毒占75．2%,为主要型别;EV-C组占12．2%,腺病毒占5．7%,EV-A组占2．4%。结论云南省EV的流行中,以EV-B组较多。

Study on the molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan province, China

OBJECTIVE: This report presented an overview on the epidemiology of enterovirus in Yunnan province, the People's Republic of China. METHODS: A total of 210 strains of non-polioviruses isolated under acute flaccid paralysis surveillance during a 5-year study period from 1997 to 2000 and 2004 were examined. Of the 210 non-polioviruses strains, a total of 12 strains of adenoviruses were serologically identified. The remaining 198 isolates were used for molecular typing, and the viral genomes of 195 nonpolio enteroviruses (NPEVs) were translated to corresponding amino acid sequences and compared with those of the prototype strains. RESULTS: Based on molecular typing, 5 isolates were classified into 5 serotypes of human enterovirus A species while 158 isolates into 34 serotypes of B and 32 isolates into 6 serotypes of C species. However, we did not isolate any viruses which belonged to human enterovirus D species. Thus, under acute flaccid paralysis surveillance, human enterovirus B species accounted for 75.2% of the 210 isolates and was considered as the predominant one, followed by human enterovirus C (12.2%), adenovirus (5.7%), and human enterovirus A (2.4%). CONCLUSION: Although the epidemiological characteristics of NPEVs from Yunnan province remained "unknown", the molecular typing method had provided us a breakthrough to understand the epidemiology of these viruses.