米非司酮对卵巢癌耐药细胞株COC1/DDP增敏作用研究

目的探讨米非司酮对卵巢癌耐药细胞株COC1/DDP的增敏作用。方法采用RPMI1640培养液对COC1/DDP细胞株进行培养,实验分2批进行,第一批将COC1/DDP细胞株分5组:对照组(不加任何药物)、顺铂组(2μg/ml)、高剂量米非司酮组(15μg/ml)、中剂量米非司酮组(10μg/ml)、低剂量米非司酮组(5μg/ml);第二批将COC1/DDP细胞株分5组:对照组(不加任何药物)、顺铂组(2μg/ml)、高剂量米非司酮加顺铂组(15μg/ml米非司酮和2μg/ml顺铂)、中剂量米非司酮加顺铂组(10μg/ml米非司酮和2μg/ml顺铂)、低剂量米非司酮加顺铂组(5μg/ml米非司酮和2μg/ml顺铂)。培养结束后采用MTT法观察各组COC1/DDP细胞株增殖活力和生存率的变化。结果与对照组比较,顺铂组和低剂量米非司酮组对细胞增殖活力的影响,差异均无统计学意义(P>0.05);随着米非司酮浓度的升高,对COC1/DDP细胞抑制作用逐渐增强,中、低剂量米非司酮组与顺铂组比较细胞增殖活力和生存率差异均有统计学意义(P<0.05或P<0.01),高剂量组与低剂量组比较有统计学意义(P<0.01);各剂量米非司酮加顺铂组与顺铂组比较对细胞增殖活力和生存率的影响,差异有统计学意义,且随着米非司酮浓度的升高,对COC1/DDP细胞抑制作用逐渐增强(P<0.05或P<0.01)。结论单药米非司酮和联合顺铂对COC1/DDP细胞增殖活力均具有显著抑制作用,米非司酮可提高顺铂化疗的敏感性,且与米非司酮的浓度呈剂量依赖关系。

The sensitization effect of mifepristione on drug resistance of ovarian cancer cell lines COC1/DDP

Objective To discuss the sensitization effect of mifepristone on drug resistance of ovarian cancer cell lines COC1/DDP.Method The cell lines COC1/DDP were cultivated by RPMI 1640 culture solution.The experiment were carried out followed two steps,the first step was to divided the cell lines COC1/DDP into five groups,included control group(without medicine),diamminedichloroplatinum(DDP)group(2 μg/ml),high-dose mifepristone group(15 μg/ml),middle-dose mifepristone group(10 μg/ml)and low-dose mifepristone group(5 μg/ml).The second step was to divided the cell lines COC1/DDP into five groups,included control group(without medicine),DDP group(2 μg/ml),high-dose mifepristone combine DDP group(15 μg/ml mifepristone and 2 μg/ml DDP),middle-dose mifepristone combine DDP group(10 μg/ml mifepristone and 2 μg/ml DDP)and low-dose mifepristone combine DDP group(5 μg/ml mifepristone and 2 μg/ml DDP);After culture,the effect of each group on COC1/DDP cell proliferation activity and survival rate were evaluated with MTT method.Result Compared with control group,the cell proliferation activity in DDP group and low-dose mifepristone group showed no statistical significance(P>0.05);otherwise,the COC1/DDP cyto-inhibition were gradually increase followed by the concentration of mifepristone.Compared with DDP group,the cell proliferation activity and survival rate in middle and low-dose mifepristone group had statistical significance(P<0.05 or P<0.01),there were statistical significance in the low-dose mifepristone group and high-dose mifepristone group(P<0.01);Compared with DDP group,the cell proliferation activity in mifepristone combine DDP group were decreased obviously,and the COC1/DDP cyto-inhibition were changed gradually following with the increase of mifepristone concentration(P<0.05 or P<0.01).Conclusion There were obvious cyto-inhibition effect on COC1/DDP cell proliferation activity with mifepristone single or combine with DDP.It demonstrated that mifepristone could improve the sensitivity of DDP,and had close relationship with the concentration of mifepristone.