三氧化二砷联合PDTC诱导胃癌细胞凋亡及其对VEGF表达的影响

目的 研究三氧化二砷(arsenic trioxide,As2O3)和吡咯烷二硫代氨基甲酸盐(pyrrolidine dithiocarbamate,PDTC)联合诱导胃癌细胞.SGC-7901凋亡及对血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响. 方法 用不同浓度的As2O3和(或)PDTC处理SGC-7901细胞,用四甲基偶氮唑蓝(MTT)法测细胞生长抑制率,用流式细胞仪(ECM)检测细胞周期及细胞凋亡,并用免疫细胞化学方法 检测VEGF的表达. 结果 As2O3可抑制SGC-7901的生长,并随浓度的增加而增强.As2O3与PDTC联合比单用As2O3或PDTC抑制效果更明显(P<0.05).As2O3>作用后SGC-7901 Cn/M期细胞比例上升,联合用药组比单用药组G2/M期细胞比例上升更明显(P<0.05).As2O3可诱导SGC-7901细胞凋亡,联合用药组比单药组组凋亡率增加更明显(P<0.05);As2O3组VEGF表达下降(P<0.05),联合用药组比单药组表达下降更明显(P<0.05). 结论 As2O3与PDTC联合应用具有显著抗胃癌作用,其机制可能与细胞周期阻滞、增强诱导胃癌细胞凋亡以及抑制NF-кB、VEGF表达有关.

Effect of As_2O_3 and PDTC on apoptosis of human gastric cancer cell line SGC-7901 and VEGF expression

Objective To explore the effect of arsenic trioxide(As2O3)combined with pyrrolidine dithiocarbamate(PDTC)on apoptosis of human gastric cancer cell line SGC-7901 and expression of vascular endothelial growth factor(VEGF). Methods SGC-7901 cells were respectively treated in vitro with As2O3 and PDTC alone or in combination.The inhibition rate of cell growth was assayed by MTT method.Cell cycles and apoptosis were analyzed by flow cytometry(FCM).The expression of VEGF was detected by immunocytochemistry. Resul...