| 内皮素-1对人系膜细胞表达MMP-3及TIMP-1的影响 |
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| 引用本文： | 黄志芳,伦立德,季康. 内皮素-1对人系膜细胞表达MMP-3及TIMP-1的影响[J]. 基础医学与临床, 2006, 26(1): 66-69 |
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| 作者姓名： | 黄志芳 伦立德 季康 |
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| 作者单位： | 1. 解放军空军总医院,肾病科,北京,100036
2. 武警北京总队医院,内二科,北京,100027 |
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| 摘 要： | 目的研究内皮素-1(ET-1)对人系膜细胞(MC)基质金属蛋白酶-3(MMP-3)及其组织抑制剂-1(TIMP-1)表达的影响。方法采用体外MC培养,应用细胞ELISA法测定MC内MMP-3、TIMP-1蛋白的表达。结果ET-1促进MC内MMP-3、TIMP-1蛋白表达,但MMP-3/TIMP-1比值呈剂量及时间依赖性下降。结论ET-1可能是人系膜细胞内MMP-3/TIMP-1比值下降的原因之一,这与肾小球细胞外基质降解受抑密切相关。
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| 关 键 词： | 内皮素-1 系膜细胞 基质金属蛋白酶 金属蛋白酶组织抑制剂 |
| 文章编号： | 1001-6325(2006)01-0066-04 |
| 收稿时间： | 2004-06-24 |
| 修稿时间： | 2005-03-14 |
Effects of endothelin-1 on the expressions of MMP-3 and TIMP-1 in human mesangial cells |
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| HUANG Zhi-fang,LUN Li-de,JI Kang. Effects of endothelin-1 on the expressions of MMP-3 and TIMP-1 in human mesangial cells[J]. Basic Medical Sciences and Clinics, 2006, 26(1): 66-69 |
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| Authors: | HUANG Zhi-fang LUN Li-de JI Kang |
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| Affiliation: | 1 .Department of Nephrology, Air Force General Hospital, Beijing 100036; 2. No.2 internal department, Beijing Murvicipal Corps Hospital, Beijing 100027, China |
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| Abstract: | Objective To investigate the effects of endothelin-1(ET-1) on the expressions of matrix metalloproteinase-3(MMP-3) and tissue inhibitor of metalloproteinase-1(TIMP-1) in human mesangial cells(MCs).Methods Cultured human MCs were exposed to ET-1,the expressions of MMP-3 and TIMP-1 proteins were measured by ELISA.Results The expressions of MMP-3 and TIMP-1 in MCs were enhanced by ET-1,but the ratio of MMP-3/TIMP-1 decreased in a dose-dependent and time-dependent manner.Conclusion ET-1 may decreased the ratio of MMP-3/TIMP-1 in MCs,which then contribute to the inhibition of ECM degradation in glomerulus. |
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| Keywords: | endothelin-1 mesangial cell matrix metalloproteinase tissue inhibitor of metalloproteinase |
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