| HPLC-ELSD法测定健脾益气胶囊中黄芪甲苷的含量 |
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| 引用本文： | 邱凯锋,李国成,张小兵. HPLC-ELSD法测定健脾益气胶囊中黄芪甲苷的含量[J]. 中国医药指南, 2011, 9(27): 14-15 |
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| 作者姓名： | 邱凯锋 李国成 张小兵 |
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| 作者单位： | 1. 中山大学孙逸仙纪念医院,广东,广州,510120
2. 广州市花城制药厂,广东,广州510555 |
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| 摘 要： | 目的建立HPLC-ELSD测定健脾益气胶囊中黄芪甲苷的含量。方法选用Hypersil BDS C18色谱柱(5μm,200mm×5.0mm),流动相是乙腈-水(32∶68),流速为1.0mL/min,检测器漂移温度是40℃,载气为3.5mL/min。结果样品浓度在0.2~2.0μg/mL之间与峰面积成良好的线性关系(r=0.9996),平均回收率为97.32%,RSD为2.31%。结论本方法简便、准确、灵敏度高,可用于该制剂质量控制。
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| 关 键 词： | 健脾益气胶囊 黄芪甲苷 HPLC-ELSD |
HPLC-ELSD Determination of Astragaloside IV in Jianpiyiqi Capsules |
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| QIU Kai-feng,LI Guo-cheng,ZHANG Xiao-bing. HPLC-ELSD Determination of Astragaloside IV in Jianpiyiqi Capsules[J]. Guide of China Medicine, 2011, 9(27): 14-15 |
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| Authors: | QIU Kai-feng LI Guo-cheng ZHANG Xiao-bing |
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| Affiliation: | 1 Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;2 Huacheng Pharmaceutical Factory of Guangzhou,Guangzhou 510555,China) |
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| Abstract: | Objective To establish an HPLC-ELSD method for the determination of astragaloside Ⅳ in Jianpiyiqi capsules.Methods The HPLC-ELSD was manufactured by Hypersil Co;the chromatographic column was BDS C18（5μm,200×5.0mm）;the mobile phase was composed of acetonitrile-methanol（32∶68） with flow rate of 1.0mL/min;the detection temperature was 40℃;the carrier gas was 3.5mL/min.Results Astragaloside Ⅳ showed good linearity（r=0.9997） in the range of 0.2-2.0μg/mL,（r=0.9996）.The average absolute recovery was 97.32%,RSD=2.31%.Conclusion The method is simple,accurate and high sensitivity.It can be used for the quality control of this capsule. |
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| Keywords: | Jianpiyiqi capsules Astragaloside Ⅳ HPLC-ELSD |
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