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| HPLC法测定宫环养血颗粒中芍药苷的含量 | |
| 引用本文： | 邓跃花. HPLC法测定宫环养血颗粒中芍药苷的含量[J]. 中国医药指南, 2011, 9(26): 33-33,35 |
| 作者姓名： | 邓跃花 |
| 作者单位： | 娄底眼科医院,湖南,娄底,417000 |
| 摘 要： | 目的建立测定宫环养血颗粒中芍药苷含量的HPLC方法。方法采用Shim-pack VP-ODS柱,乙腈-0.05%磷酸水溶液(16∶84)为流动相,检测波长为230nm,流速1.0mL/min。结果芍药苷在0.0476~0.912μg范围内线性关系良好,r=0.9996,平均回收率为99.51%,RSD为1.35%(n=6)。结论该方法简便、准确、重现性好,可用于宫环养血颗粒的质量控制。 |
| 关 键 词： | 宫环养血颗粒 芍药苷 高效液相色谱法 |
Determination of Paeoniflorin in Gonghuan Yangxue Granule by HPLC |
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| DENG Yue-hua. Determination of Paeoniflorin in Gonghuan Yangxue Granule by HPLC[J]. Guide of China Medicine, 2011, 9(26): 33-33,35 | |
| Authors: | DENG Yue-hua |
| Affiliation: | DENG Yue-hua (Loudi Eye Hospital, Loudi 417000, China) |
| Abstract: | Objective To establish a HPLC for determination of Paeoniflorin in Gonghuan Yangxue Granule. Method A Shim-pack VP-ODS column was used. The mobile phase was acetonitrile-0.05%phosphate （ 16 ： 84） , the detecting wavelength was at 230nm, the flow rate was 1.0mL/min. Result A good linearity of Paeoniflorin was in the range of 0.0476-0.9121ag, r=0.9996, the average sample recovery was 99.51% with RSD of 1.35% （n=6） . Conclusion This method has good repeatability and flexibility. It can be used for quality control of Gonghuan Yangxue Granule. |
| Keywords: | Gonghuan Yangxue Granule Paeoniflorin HPLC |
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