Snail介导的EMT在上皮性卵巢癌组织中的作用

目的：检测Snail、E-cadherin及Vimentin在上皮性卵巢癌组织中的表达，探讨Snail介导的上皮间质转化（EMT）在卵巢癌发生、发展及转移中的作用。方法采用免疫组化法分别检测Snail、E-cadherin、Vimentin在48例卵巢浆液性腺癌、卵巢交界性浆液性肿瘤及卵巢浆液性腺瘤中的表达，探讨EMT相关因子表达强度的相关性及与临床病理特征之间的关系。结果（1）Snail、Vimentin在卵巢浆液性腺癌中的表达率为（68.75%/66.67%），高于卵巢交界性浆液性腺瘤的（41.67%/37.50%）及卵巢浆液性腺瘤的（25.00%/18.75%），结果均具有显著的统计学差异（P＜0.05），E-cadherin在卵巢浆液性腺癌中的表达率为27.08%，低于与卵巢交界性浆液性腺瘤的54.17%及卵巢浆液性腺瘤的75.00%，结果均具有统计学差异（P＜0.05）。而卵巢交界性浆液性腺瘤组与卵巢浆液性腺瘤组比较，三种蛋白的表达均无统计学差异（P＞0.05）。（2）Snail、E-cadherin及Vimentin的表达高低与FIGO分期、分化级别、有无淋巴结转移及腹膜种植有关。（3）在卵巢浆液性腺癌中，Snail与Vimentin的表达呈正相关（r=0.477，P＜0.05），Snail与E-cadherin的表达呈负相关（r=-0.601，P＜0.05），而E-cadherin与Vimentin表达的相关性不明显（r=-0.206, P＞0.05）。结论在上皮性卵巢癌组织中，Snail、Vimentin表达上调，E-cadherin表达下调，提示Snail介导的EMT可能与卵巢癌的发生、发展及浸润转移有关。

Effects of Snail mediated EMT in epithelial ovarian carcinoma

Objective To Study the effect on Snail mediated epithelial mesenchymal transition （EMT） in ovarian cancer occurrence, development and metastasis. Methods Immunohistochemical staining was detected Snail, E-cadherin,in 48 cases of ovarian serous adenocarcinoma,ovarian serous borderline tumors and ovarian serous adenomas Vimentin,investigate the correlation between the expression of EMT -related factors and the strength and relationship between clinicopathological features. Results（1）The expression of Snail,Vimentin in ovarian serous adenocarcinoma was 68.75% and 66.67%,higher than in borderline serous ovarian tumors （41.67% and 37.50） and in ovarian serous adenomas（ 25.00%and 18.75%）,with significant difference（P〈0.05）,the expression of E-cadherin in ovarian serous adenocarcinoma of the rate of 27.08%,lower than in borderline serous ovarian tumors（54.17%） and in ovarian serous adenomas（75.00%）,with significant difference（P 〈 0.05）. Between the borderline ovarian serous cystadenoma and ovarian serous adenoma group,there was statistical difference in the expression of the three proteins（P ＆gt; 0.05）.（2）The expression level of Snail and FIGO, E-cadherin and Vimentin staging,differentiation level,there was no lymph node metastasis and peritoneal implants.（3）in ovarian serous adenocarcinoma, the expression of Snail positively correlated with Vimentin （r=0.477,P 〈 0.05）,the expression of Snail was negatively correlated with E-cadherin（r=-0.601,P 〈 0.05）,and the expression of E-cadherin and Vimentin correlation is not obvious（r=-0.206,P〈 0.05）. Conclusion In epithelial ovarian cancer,Snail,Vimentin upregulation,E-cadherin downregulation,suggesting that Snail-mediated EMT may be transferred with ovarian cancer,development and infiltration.