阿司匹林诱导人宫颈癌HeLa细胞凋亡及机制

目的:探讨阿司匹林对人宫颈癌HeLa细胞凋亡的作用及机制。方法:体外培养HeLa细胞,分别以0.5,1.0,5.0mmol.L-1阿司匹林干预。采用Annexin V-FITC/PI凋亡试剂盒检测凋亡;碘化丙锭(PI)染色法检测细胞周期;Western-blot法检测NF-κB p65蛋白表达。结果:0.5,1.0,5.0mmol.L-1的阿司匹林均能使Hela细胞凋亡率明显增加,S期、G2/M期细胞比例减少,G0/G1期细胞比例显著增加,细胞中NF-κB p65蛋白表达水平明显下降,且上述作用均呈剂量依赖性。结论:阿司匹林在体外可以通过下调NF-κB p65蛋白表达,影响细胞周期,诱导Hela细胞凋亡。

Effect and mechanism of action of aspirin induced apoptosis of human cervical cancer cell lines Hela

OBJECTIVE To study the regulatory effect of aspirin on the cell apoptosis of human cervical cancer cell lines Hela and its molecular mechanism of action.METHODS Hela cells were cultured in vitro and treated with aspirin at the concentration of 0.5,1.0,5.0 mmol·L-1respectively.The rate of apoptosis and cell cycle were analyzed by flow cytometry analysis(FACS).Western-blot was performed to examine the expression of NF-κB p65 protein.RESULTS 0.5,1.0,5.0 mmol·L-1 aspirin significantly increased the rate of apoptosis and the proportion of cells in G0/G1 phase and decreased the proportion of cells in S and G2/M phases in a dose-depending manner.With the increasing of aspirin concentration,the expression of NF-κB p65 was gradually downregulated.CONCLUSION Aspirin can regulate cell cycle distribution and accelerate cell apoptosis through inhibiting the expression of NF-κB p65 protein in cell lines Hela.