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| 乙醇醛对小鼠脾淋巴细胞DNA损伤作用的研究 | |
| 引用本文： | 梁雅静,李才,侯芳玉. 乙醇醛对小鼠脾淋巴细胞DNA损伤作用的研究[J]. 卫生研究, 2004, 33(2): 214-215 |
| 作者姓名： | 梁雅静 李才 侯芳玉 |
| 作者单位： | 吉林大学再生医学科学研究所,长春,130021 |
| 基金项目： | 吉林省科技发展计划项目(No.20010514) |
| 摘 要： | 目的 观察乙醇醛对DNA的损伤作用。方法 采用单细胞凝胶电泳 (SCGE)技术检测不同浓度乙醇醛对小鼠脾淋巴细胞DNA的损伤程度。结果 当乙醇醛浓度为 0 0 1mmol L时即能引起脾淋巴细胞DNA链断裂损伤 ,DNA损伤率为 8% ,DNA平均迁移距离为 ( 14 32± 2 84 ) μm。随着乙醇醛浓度的升高DNA损伤率和DNA平均迁移距离均以浓度依赖方式增加。结论 乙醇醛可引起细胞DNA损伤 |
| 关 键 词： | 乙醇醛 单细胞凝胶电泳 DNA损伤 |
| 文章编号： | 1000-8020(2004)02-0214-02 |
| 修稿时间： | 2003-09-25 |
Study of glycolaldehyde on DNA damage in mouse spleen lymphocytes |
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| Yajing Liang,Cai Li,Fangyu Hou. Study of glycolaldehyde on DNA damage in mouse spleen lymphocytes[J]. Journal of hygiene research, 2004, 33(2): 214-215 | |
| Authors: | Yajing Liang Cai Li Fangyu Hou |
| Affiliation: | Institute of Frontier Medical Science, Jilin University, Changchun 130021, China. |
| Abstract: | OBJECTIVE: Study the effect of glycolaldehyde on DNA of mouse spleen lymphocytes. METHODS: The DNA damage induced by different concentrations of glycolaldehyde was detected using single cell gel electrophoresis assay (SCGE). RESULTS: Glycolaldehyde at 0.01 mmol/L could induce lymphocyte DNA strand breakage and DNA damage rate was 8% and, the average migration distance of DNA was (14.32 +/- 2.84) micron. DNA damage rate and the average migration distance of DNA were increased in a concentration-dependent manner. CONCLUSION: glycolaldehyde could induce DNA damage in different concentration in this experiment. |
| Keywords: | glycolaldehyde single cell gel electrophoresis DNA damage |
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