| 白念珠菌烯醇化酶的抗原特异性研究 |
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| 引用本文： | 唐宁枫,焦瑞清,吴绍熙,郭宁如,秦浚川.白念珠菌烯醇化酶的抗原特异性研究[J].中华皮肤科杂志,2001,34(5):340-342. |
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| 作者姓名： | 唐宁枫 焦瑞清 吴绍熙 郭宁如 秦浚川 |
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| 作者单位： | 1. 中国医学科学院、中国协和医科大学真菌中心,
2. 南京大学医药生物技术国家重点实验室 |
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| 基金项目： | 卫生部重点项目 (97010211)；南京大学医药生物技术国家重点实验室开放基金资助 (XW199902) |
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| 摘 要： | 目的 探讨白念珠菌烯醇化酶的抗原特异性。方法和结果 （1）免疫印迹显示烯醇化酶是白念珠菌非糖基化蛋白中可以和感染兔血清发生反应的主要蛋白质。（2）以纯化的白念珠菌烯醇化酶免疫家兔获得最高效价为1：6400的多克隆抗体（ELISA法测定）。（3）与另外3种多克隆抗体相比，抗烯醇化酶抗体在检测白念珠菌菌体抗原时显示出较高的特异性。结论 白念珠菌烯醇化酶是一种值得进一步研究的特异性抗原。
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| 关 键 词： | 白色念珠菌 烯醇化酶 抗原 |
| 修稿时间： | 2001年1月3日 |
Study on Antigenic Specificity of Candida albicans Enolase |
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| TANG Ningfeng,JIAO Ruiqing,WU Shaoxi,et al..Study on Antigenic Specificity of Candida albicans Enolase[J].Chinese Journal of Dermatology,2001,34(5):340-342. |
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| Authors: | TANG Ningfeng JIAO Ruiqing WU Shaoxi |
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| Institution: | TANG Ningfeng,JIAO Ruiqing,WU Shaoxi,et al.Institute of Dermatology,Chinese Academy of Medical Scie nces,Peking Union Medical College,Nanjing210042,China |
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| Abstract: | Objective To evaluate the antigenic specificity of the enolase.Methods and Results The results included:(1)Western blot analysis demonstrated that enolase was a main protein in the nonglyco-proteins of C.albicans which could react with the serum of C.albicans infected rabbit.(2)The purified enolase was used to immunize rabbit for producing anti-enolase serum,and the hig hest titer of polyclonal antibody against enolase was up to 1:6400with enzyme-linked immunoadso rbent assay(ELISA).(3)Compared with three other polyclonal antibodies,anti-enola se antibody showed high specificity in detecting C.albicans antigen.Conclusion Enolase as a specific antigen,is worthy of further investigation in C.albicans cell. |
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| Keywords: | Candida albicans Enolase Antigens |
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