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Antifibrosis effects of total glucosides of Danggui-Buxue-Tang in a rat model of bleomycin-induced pulmonary fibrosis
Authors:Gao Jian  Huang Yan  Li Ping  Xu Dujuan  Li Jun  Liu Yong  Huang Zhaogang  Wu Qiang  Shao Xu
Affiliation:a Pharmaceutical preparation section, Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine(TCM-2009-202), the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China
b School of Pharmacy, Anhui Medical University, Hefei 230032, Anhui, China
c Key Laboratory of Modern Chinese Medicines (Ministry of Education), China Pharmaceutical University, Nanjing 210009, China
d Department of Pathology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China
e Hefei Seven Star Medicine and Technology Co. Ltd., Hefei 230088, Anhui, China
Abstract:

Aim of the study

The present study examined the antifibrosis effects of DBTG (total glucosides of Danggui-Buxue-Tang) on bleomycin-induced pulmonary injury and fibrosis in rats.

Materials and methods

Animals were randomly divided into six groups: (1) saline control group; (2) Bleomycin group in which rats were endotracheally instillated with bleomycin (5 mg/kg); (3-5) Bleomycin and DBTG group, in which DBTG were given to rats daily (16.32 or 64 mg/kg/day, i.g.) one day after bleomycin injection for 4 weeks until the end of the treatment; (6) Bleomycin and positive control group. Animals were sacrificed at 7, 14, and 28 days post bleomycin administration and lungs were removed. Lung specimens were stained with hematoxylin and eosin (HE) and Masson trichrome for histological evaluation of lung injury and fibrosis by light microscopy. Body weight and lung index from various groups were measured, as well as TNF-α, TGF-β1 and type I collagen concentrations in lung homogenates.

Results

DBTG reduced bleomycin-induced weight loss, decreased the lung index and histological evidence supported the ability of DBTG to attenuate bleomycin-induced lung fibrosis and consolidation. DBTG could partly dose-dependently decrease TNF-α and TGF-β1 activity, as well as it decrease type I collagen expression in lung tissues.

Conclusions

The findings of the present study provide evidence that DBTG may serve as a novel target for potential therapeutic treatment of lung fibrosis.
Keywords:Total glucosides of Danggui-Buxue-Tang   Pulmonary fibrosis   TGF-β1   TNF-α   Type I collagen
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