| 核酸对淋巴细胞DNA损伤修复的影响 |
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| 引用本文： | 潘洪志,陈文华,李辉,欧阳吉芳,李恕. 核酸对淋巴细胞DNA损伤修复的影响[J]. 实用预防医学, 2005, 12(6): 1295-1296 |
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| 作者姓名： | 潘洪志 陈文华 李辉 欧阳吉芳 李恕 |
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| 作者单位： | 1. 哈尔滨医科大学公共卫生学院,黑龙江,哈尔滨,150001
2. 珍奥集团股份有限公司 |
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| 摘 要： | 目的 研究核酸对大鼠淋巴细胞DNA损伤的保护作用。方法 纯种雄性健康Wistar大鼠，随机分为4组：空白组、模型组、核酸低剂量组、核酸高剂量组。空白组正常饮水，其余3组饮0．8％醋酸铅水溶液，核酸组灌胃核酸，空白组、模型组灌蒸馏水，5周后，采用单细胞凝胶电泳技术对外周血淋巴细胞DNA损伤进行分析。结果 模型组大鼠淋巴细胞DNA的迁移率和尾长显著高于空白组，核酸组大鼠淋巴细胞DNA的迁移率和尾长显著低于模型组，在统计学上差异有显著性（P〈0．05）。结论 慢性铅染毒可致大鼠淋巴细胞DNA损伤，饮食核酸对淋巴细胞DNA损伤具有一定的保护作用，可减轻铅中毒引起的氧化损伤。
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| 关 键 词： | 核酸 淋巴细胞 DNA损伤 单细胞凝胶电泳 |
| 文章编号： | 1006-3110（2005）06-1295-02 |
| 收稿时间： | 2005-07-27 |
| 修稿时间： | 2005-07-27 |
Effect of Dietary Nucleic Acid on DNA Oxidative Damage in Peripheral Blood Lymphocytes in Rats |
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| PAN Hong-zhi,CHEN Wen -hua, LI Hui,et al.. Effect of Dietary Nucleic Acid on DNA Oxidative Damage in Peripheral Blood Lymphocytes in Rats[J]. Practical Preventive Medicine, 2005, 12(6): 1295-1296 |
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| Authors: | PAN Hong-zhi CHEN Wen -hua LI Hui et al. |
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| Affiliation: | School of Public Health, Harbin Medical University, Harbin 150001, Heilongjiang, China |
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| Abstract: | Objective To test the protective effect of dietary nucleic acid on lymphocyte DNA damage repair. (Methods) Inbreed healthy male Wistar rats were experimentally exposed to lead acetate to induce DNA injury and received dietary nucleic acid to test their protective effects on lymphocyte DNA damage repair.After 5 weeks experimental treatment,the DNA breaks in peripheral blood lymphocytes were analyzed with single cell gel electrophoresis. Results Compared(with) control,the DNA damage in dietary nucleic acid supplemented groups were reduced significantly(P<0.05). (Conclusion) (Dietary) nucleic acid could protect DNA damage of peripheral blood lymphocytes in rats. |
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| Keywords: | Nucleic Acid Lymphocyte DNA damage Single cell gel electrophoresis |
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