菟丝子总黄酮对大鼠流产模型母-胎界面Fas／FasL、PCNA、HB—EGF表达的影响

目的:观察菟丝子总黄酮对大鼠流产模型母-胎界面Fas/FasL、PCNA、HB-EGF表达的影响。方法:对溴隐亭致SD孕鼠流产模型,以菟丝子总黄酮高、低剂量为治疗组,达芙通为阳性对照组,于孕12 d处死各组大鼠,应用免疫组织化学方法检测胎盘和蜕膜Fas/FasL、PCNA、HB-EGF的表达。结果:菟丝子总黄酮高、低剂量组、达芙通及正常妊娠组蜕膜和胎盘PCNA表达显著高于模型组,蜕膜和胎盘Fas/FasL表达显著低于模型组,胎盘HB-EGF表达显著高于模型组,差异均有显著性,菟丝子总黄酮高、低剂量组与孕激素达芙通组比较,差异无显著性,各组蜕膜皆无HB-EGF表达。结论:菟丝子总黄酮可能通过调节滋养细胞的增殖与凋亡,使其恢复到正常妊娠状态而起到保胎的作用。

Effect of Total Flavones from Cuscuta chinensis on Expression of Fas/FasL,PCNA and HB-EGF in SD Rats Model with Bromocriptine-induced Abortion

Objective :To explore the effect of total flavones from cuscuta chinensis(TFCC) on expression of Fas,PCNA and HB-EGF in SD rats model with bromocriptine-induced abortion.Methods:The model rats of bromocriptine during 6-8 d of pregnancy induced early abortion was established,adopting respectively herbs in high and low dosage and progesterone affect model rat and after 12d,Immunohistochemical was applied to determine Fas,HB-EGF and PCNA in deciduas and placenta.Results:Expression of PCNA on trophoblast and deciduas,HB-EGF on trophoblast,PR on deciduas in the model used Semen cuscutae flavonoid,proesterone and normal pregnacy,were significantlly higher than those of the pure model.Expression of Fas on trophoblast and deciduas in above four groups,were significantlly lower than those of the pure model.There were no expression of HB-EGF on deciduas.Conclusion:TFCC regulates the proliferation and apoptosis of the deciduas and cytotrophoblasts and prevents spontaneous abortions.