核苷酸结合寡聚化结构域样受体蛋白3和caspase-1表达与非小细胞肺癌患者疾病进展的关系

目的 观察非小细胞肺癌(non-small cell lung cancer,NSCLC)患者外周血核苷酸结合寡聚化结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3,NLRP3)及血清caspase-1 的表达情况,探...

Correlations of NLRP3and caspase-1levels with the progression of non-small cell lung cancer

Objective To analyze the relationships of the expression of nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)in peripheral blood and serum caspase-1level with the disease progression by observing the expressions of NLRP3and caspase-1in patients with non-small cell lung cancer(NSCLC).Methods Totally 252patients with NSCLC received GP(gemcitabine+cisplatin)regimen chemotherapy and their efficacies were evaluated after 2 cycles,in which 125patients reached the clinical control and were divided into progressive group(n=102)and non-progressive group(n=23)according to 2-year follow-up result.The expressions of NLRP3in peripheral blood and the serum levels of caspase-1,P53and vascular endothelial growth factor(VEGF)were compared between two groups.ROC was drawn to evaluate the efficacies of NLRP3and caspase-1on predicting the progression of NSCLC after GP chemotherapy.COX regression analysis was used to assess the effects of NLRP3and caspase-1on the progression of NSCLC in patients responding to GP chemotherapy.Results The levels of NLRP3mRNA,caspase-1,P53and VEGF were higher in progression group(2.00(1.92,4.02),296.56(263.52,360.75)ng/L,8.12(6.32,9.41)u/mL,398.13(341.12,426.59)ng/L)than those in non-progressive group(1.36(0.86,1.75),196.56(141.11,211.32)ng/L,3.68(3.12,4.87)u/mL,289.42(245.16,311.32)ng/L)(P<0.05).When the optimal cut-off values of NLRP3mRNA and caspase-1were 1.361and 143.215ng/L,the AUCs for predicting the disease progression were 0.859(95%CI:0.771-0.947,P<0.001)and 0.851(95%CI:0.751-0.951,P<0.001).NLRP3mRNA≥1.361and caspase-1≥143.215ng/L were the independent risk factors of progression of NSCLC in patients responding to GP chemotherapy(OR=2.696,95%CI:1.627-5.468,P<0.001;OR=3.377,95%CI:2.034-5.606,P<0.001).Conclusion The progression of NSCLC after GP chemotherapy may be correlated with the overexpressions of NLRP3in peripheral blood and serum caspase-1.The detection of NLRP3 and caspase-1 levels of patients with NSCLC contributes to the prediction of disease progression after chemotherapy,the guidance of the treatment of NSCLC and the improvement of prognosis.