| RP-HPLC法测定莲子心提取物中去甲乌药碱的含量 |
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| 引用本文： | 惠战锋,王前刚,魏谈笑,等.RP-HPLC法测定莲子心提取物中去甲乌药碱的含量[J].西北药学杂志,2014(1):14-16. |
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| 作者姓名： | 惠战锋 王前刚 魏谈笑 等 |
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| 作者单位： | [1]陕西嘉禾植物化工有限责任公司,西安710086 [2]西北大学生命科学学院,西安710069 |
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| 摘 要： | 目的建立测定莲子心提取物中去甲乌药碱的HPLC分析方法。方法色谱柱为Intersil ODS-3C18(150mm×4.6mm,5μm);流动相为乙腈-水-磷酸(450∶700∶1,其中含有4.6g十二烷基硫酸钠);检测波长为284nm;进样量为10μL;体积流量为1.0mL·min-1;柱温30℃。结果去甲乌药碱的线性范围为32.92164.6μg·mL-1,r=0.999 9(n=5),平均回收率为98.0%,RSD为0.51%。结论该方法简单、准确、重复性好,可用于莲子心提取物中去甲乌药碱的定量测定。
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| 关 键 词： | HPLC 莲子心提取物 去甲乌药碱 |
Determination of higenamine in Nelumbo nucifera extract by RP-HPLC |
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| HUI Zhanfeng,WANG Qiangang,WEI Tanxia,LI Duowei.Determination of higenamine in Nelumbo nucifera extract by RP-HPLC[J].Northwest Pharmaceutical Journal,2014(1):14-16. |
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| Authors: | HUI Zhanfeng WANG Qiangang WEI Tanxia LI Duowei |
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| Institution: | 1. Shaanxi Jiahe Phytochem Limited Company, Xi＇an 710086; 2. School of Life Sciences,Northwest University, Xitan 710069, China) |
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| Abstract: | Objective To establish an HPLC method for the determination of higenamine in Nelumbonucifera extract.Methods The test was carried out on an Intersil ODS-3 C18 column（150 mm×4.6 mm,5μm）,with acetonitrile-water-phosphoric acid （450∶700∶1,4.6 g of lauryl sodium sulfate was dissolved in mobile phase）as the mobile phase.The detection wavelength was 284 nm, sample quantity was 10μL,and the flow rate was 1.0 mL·min-1 .The column temperature was 30 ℃.Results The calibration curves were linear in the range of 32.92-164.6 μg·mL-1 ,r=0.999 9 （n=5）,and the average recovery of higenamine was 98.0%,RSD 0.51%.Conclusion The method is simple,accurate,reproducible and could be used for the determination of the content of higenamine in Nelumbo nucifera extract. |
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| Keywords: | RP-HPLC Nelumbo nucifera extract higenamine |
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