早期胃癌活体组织中血管内皮生长因子、p53蛋白和增殖细胞核抗原表达与预后的关系

目的研究早期胃癌患者术前胃镜活检组织中血管内皮生长因子(VEGF)、p53蛋白和增殖细胞核抗原(PCNA)表达与胃癌预后的关系.方法对1980～1991年68例早期胃癌患者的术前胃镜活检标本做VEGF、p53蛋白和PCNA免疫组化染色.结果胃癌复发组的VEGF阳性率(5/5)显著高于无复发组(14/63),P＜0.01;VEGF阳性胃癌患者的8年生存率(14/19)显著低于VEGF阴性胃癌患者的生存率(49/49),P＜0.01;p53蛋白和PCNA表达在2组中差异无显著意义,P＞0.05.结论胃镜活检组织中VEGF的表达对于早期胃癌患者是重要的预后因素,可指导术前术式的选择.

The prognostic significance of VEGF, p53 and PCNA expression in endoscopic biopsy specimens of patients with early gastric carcinoma

Objective To study the correlation between postoperative tumor recurrence and VEGF expression in preoperative biopsy specimens of early gastric carcinoma together with p53 gene abnormality and tumor cell proliferation. Methods Sixty-eight endoscopically biopsied specimens obtained preoperativelly from patients with early gastric carcinoma were studied immunohistochemically. Date was processed by computer image analysis system. Result The rate of VEGF positivity was significantly higher in patients (n=5) with postoperative tumor recurrence than in those (n=63) without (5/5 vs. 14/63, P<0.01). The eight-year survival rate of patients with positive staining of anti-VEGF antibodies was significantly lower than that negative (14/19 vs. 49/49, P<0.01), whereas no significant correlation was found between recurrence and expression of p53 or PCNA. Conclusions VEGF expression in endoscopically biopsied specimens may be a useful prognostic factor for patients with early gastric carcinoma, and it may be useful in the prediction of early gastric carcinoma patients with high tendency of postoperative recurrence.