| 高效液相色谱法测定苦参素中相关成分方法学研究 |
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| 引用本文： | 刘炳茹,周红城,王伟. 高效液相色谱法测定苦参素中相关成分方法学研究[J]. 时珍国医国药, 2001, 12(1): 27-28 |
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| 作者姓名： | 刘炳茹 周红城 王伟 |
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| 作者单位： | 内蒙古自治区药品检验所, |
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| 摘 要： | 目的建立一种高效液相色谱法测定苦参素中有关物质的方法。方法采用C18柱，流动相0.05mol/L磷酸二氢钠-乙腈-甲醇-高氯酸钠(910∶50∶10∶20)。检测波长220nm。柱温(40±1)℃。结果线性范围0.02～0.8μg，r=0.9995。结论该法分离效果好，准确可行。
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| 关 键 词： | 高效液相色谱法 苦参素 氧化苦参碱 氧化槐果碱 中药 |
| 文章编号： | 1008-0805(2001)01-0027-01 |
| 修稿时间： | 2000-08-11 |
The Methodology Study onDetermining Contents in Kushensu by HPLC |
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| LIU Bing ru,ZHOU Hong cheng,WANG Wei. The Methodology Study onDetermining Contents in Kushensu by HPLC[J]. Lishizhen Medicine and Materia Medica Research, 2001, 12(1): 27-28 |
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| Authors: | LIU Bing ru ZHOU Hong cheng WANG Wei |
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| Abstract: | Objective: To establish the determination methods of relatedsubstances in Kushensu with HPLC. Methods: Using C18 column. The mobile phase consisted of 0.05 mol/L sodium dihydrogen phosphate-acelonitrile-methanol-sodium perchlorate (910∶50∶10∶20). The UV detection wavelength was 220 nm. Temperature: (40±1)℃. Result: The standard curve was linear in the range of 0.02～0.8 μg of oxymatrine, γ=0.999 5. Conclusion: The method is accurate、 reliable and satisfactory in the isolating effect. |
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| Keywords: | HPLC Kushensu Oxymatrine Oxysophocarpin |
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