XRCC1基因多态性与宫颈癌危险性的研究

目的:探讨XRCC1基因多态性与江苏人群宫颈癌易感性之间的关系.方法:采用基于医院的分子流行病学病例对照研究方法,选取436例经组织病理学确诊为宫颁癌的新发患者作为病例组和503例年龄(±5岁)、性别相匹配的非肿瘤者作为对照组;采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法对XRCC1启动子区-77T>C和外显子10区的Arg399Glu基因多态性进行基因分型,比较不同基因型携带者患宫颈癌的危险性;通过分层分析探讨初潮年龄、患者年龄及产次对罹患宫颈癌的影响.结果:与XRCC1-77TT相比,-77TC/CC基因型可减少罹患宫颈癌的危险性(OR=0.64,95%CI=0.48～0.86).携带3～4个危险等位基因者比携带1～2个等位基因者患官颈癌的危险性更大(OR=1.44,95%CI=1.01～2.04).分层分析结果显示,年龄较大和产次较多且携带3～4个危险等位基因者罹患宫颈癌的危险性分别增加1.64倍(95%CI=1.02～2.64)和1.66倍(95%CI=1.01～2.72).本研究未发现XRCC1Arg399Glu多态性与宫颈癌之间存在显著性相关.结论:XRCC1基因启动子-77T>C多态性显著降低江苏地区汉族人群罹患宫颈癌的危险性.

The relationship between XRCC1 polymorphisms and the risk of cervical cancer in Jiangsu population

Objective:To explore the association of XRCC1 gene single nucleotide polymorphisms and the risk of cervical cancer. Methods:In a hospital-based case-control study of 436 patients with newly diagnosed cervical carcinoma and 503 cancer-free controls frequency-matched by age(± 5 years), we genotyped the -77T>C and Arg399Glu polymorphisms of XRCC1 gene by the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method. We further analyzed the interaction of age, menarche age, and parity. Results:We found that the -77T>C variant genotypes were associated with significantly decreased risk of cervical cancer adjusted odds ratio(OR) = 0.64; 95% confidence interval(CI)=0.48~0.86, compared with the wild-type homozygote. Moreover, individuals with 3~4 risk alleles increased the risk of cervical cancer accompanied with older age( > 50 years)(OR=1.64, 95%CI=1.02~2.64) and parity(OR=1.66,95%CI=1.01~2.72). By contrast, no significant association was observed between the Arg399Gln polymorphism and cervical cancer risk. Conclusion:Our finding indicated that the XRCC1-77 CC/CT genotype was significantly associated with a decreased risk of cervical cancer in Jiangsu population.