糖尿病性视网膜前膜的组织培养及其收缩性

Growth and contractility of cells from diabetic pre-retinal membrane in primary tissue culture

Cells grew out in tissue culture from 7 out of 12 diabetic pre-retinal membrane specimens. Cell types of epithelium, glia, macrophage, blood vessel, and fibroblast-like cells were distinguished by inverted phase microscopy, electron-microscopy and immunostaining. Electron-microscopy demonstrated abundant cytoplasm fibrils in some of the cells, and immunostaining for actin showed great variation in the intensity of staining. An Mg++ ATP contraction assay on 81 detergent-treated cells revealed a 25% average reduction in the cell area. Immunostaining for cytokeratin and GFAP following the Mg++ ATP assay showed no significant difference in cell contraction among epithelial, glial and other cells. The role of fibroblast-like cells and the contraction of pre-retinal membrane were discussed.