qRT-PCR法用于检测模拟环境表面载体HBV DNA的可行性研究

目的 探讨实时荧光定量PCR法(qRT-PCR法)用于检测模拟环境表面载体乙型肝炎病毒(HBV)DNA的可行性.方法 用4种浓度的HBV标准品5×106、5×105、5×104、5×103 IU/mL污染载体,放置3 d和7 d,采用qRT-PCR法,检测HBV载体表面HBV DNA浓度.结果 5×103 IU/mL标...

Feasibility study of using qRT-PCR in detection HBV DNA on simulated environmental surface carrier

Objective To explore the feasibility of using the real time fluorescent quantitative PCR(qRT-PCR)in detection of hepatitis B virus(HBV)DNA on simulated environmental surface carrier.Methods HBV standards at different concentrations of 5×106,5×105,5×104 and 5×103 IU/mL were used to contaminate the carriers,using the qRT-PCR to detect the HBV DNA on the carriers after 3 and 7 days.Results The positive rate was 70.0%and 80.0%after 3 days and 7 days on the carriers contaminated by 5×103 IU/mL of HBV standard.The rates were all 100.0%on other carriers after 3 and 7 days contaminated by the other three concentrations of standard substance.The detect concentrations were statistically significant different in four concentrations of HBV carriers after 3 days of contamination(F=8.327,P<0.001).The detect concentrations were statistically significant difference in four concentrations HBV carriers after 7 days of contamination(F=7.302,P=0.001).There were no statistically significant differences between the HBV DNA concentration reductions in four concentrations of HBV carriers after 3 and 7 days(P>0.05).Conclusion The qRT-PCR method can be used to the detect HBV contamination on the environmental surface in medical institutions.The higher the concentration of HBV contamination,the higher the detection concentration of HBV DNA.The HBV DNA on carriers can still be detected after 7 days of contamination.