过表达miR-140-5p对高脂饲养诱导的非酒精性脂肪性肝病大鼠的作用及机制

目的 探究过表达miR-140-5p对高脂饲养诱导的非酒精性脂肪性肝病(NAFLD)大鼠的作用及机制.方法 40只大鼠随机分为对照组、模型组、空载体组和过表达组,每组各10只.对照组给予普通饲料饲养,模型组给予高脂饲料饲养构建NAFLD大鼠模型,空载体组在模型组基础上于饲养第13周、第18周尾静脉注射含空载质粒的慢病毒...

Effect and mechanism of miR-140-5p overexpression on rats with non-alcoholic fatty liver disease induced by high-fat feeding

Objective This paper aims to investigate the effect and mechanism of miR-140-5p overexpression on rats with non-alcoholic fatty liver disease(NAFLD)induced by high-fat feeding.Methods Forty rats were randomly assigned to the control group,the model group,the empty vector group,and the overexpressed group,with 10 rats in each group.The control group was fed with ordinary diet,the model group was fed with high-fat diet to construct the NAFLD rat model,while the empty vector group was injected with lentivirus containing empty plasmid through tail vein at the 13th and 18th week based on the model group,and the overexpressed group was injected with lentivirus containing the overexpressed miR-140-5p plasmid through tail vein at the 13th and 18th week based on the model group.The levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay.The toll like receptor 4(TLR4)and nuclear factorκB(NF-κB)in the liver were detected by Western blot.The levels of miR-140-5p were detected by real-time fluorescence quantitative PCR.Results Compared with the control group,the relative expression of miR-140-5p in the model group was reduced,and the levels of AST,ALT,TC,TG,LDL-C,IL-6,TNF-α,TLR4,and NF-κB were increased(P<0.05).Compared with the model group and the empty vector group,the relative expression of miR-140-5p was increased in the overexpression group,and the levels of AST,ALT,TC,TG,LDL-C,IL-6,TNF-α,TLR4,and NF-κB were reduced,with statistically significant differences(P<0.05).Conclusions Overexpression of miR-140-5p can improve liver function and correct lipid metabolism disorders in NAFLD rats.The mechanism may be related to the regulation of TLR4 and NF-κB expression.