| CAR-T细胞构建技术研究进展 |
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| 引用本文： | 张苹,翟建昭,刘在栓,赵旭东. CAR-T细胞构建技术研究进展[J]. 国际检验医学杂志, 2021, 42(9): 1125-1129 |
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| 作者姓名： | 张苹 翟建昭 刘在栓 赵旭东 |
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| 作者单位： | 四川大学华西医院 实验医学科,四川成都 610041;四川大学华西医院 肿瘤动物模型创制及应用研究室,四川成都 610041 |
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| 基金项目： | 四川省科技厅项目(2020YFS0125);四川省成都市科技局项目(2019-GH02-00006-HZ、2019-YF05-00463-SN);四川大学华西医院学科卓越发展1·3·5工程临床研究孵化项目(2020HXFH038)。 |
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| 摘 要： | 嵌合抗原受体T细胞免疫疗法(CAR-T)被认为是最有潜力的癌症治疗方法,并取得了良好的临床疗效.随着CAR-T大规模应用,该技术的局限性也逐渐暴露出来,如治疗效果差 、疾病复发 、不良反应多等.这使得制造结构更优 、安全性更高 、效能更佳的CAR-T细胞成为临床所需.了解CAR-T的制造过程,掌握技术进展,是构建理想的...
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| 关 键 词： | 嵌合抗原受体T细胞免疫疗法 嵌合抗原受体 构建 基因转导 |
Advances in chimeric antigen receptor T cell immunotherapy construction technology |
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| Affiliation: | (Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Department of Tumor Animal Model Creation and Application,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China) |
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| Abstract: | Chimeric antigen receptor T cell immunotherapy(CAR-T)is considered to be the most promising treatment method and has achieved good clinical efficacy.With the large-scale clinical application of CAR-T,the limitations of this technology are gradually exposed,such as poor therapeutic effect,disease recurrence and a large number of adverse reactions.This makes the manufacture of CAR-T cells with better structure,higher safety and better efficiency clinically necessary.Understanding the manufacturing process of CAR-T and mastering the technological advances is the key to construct ideal CAR-T cells.This review focuses on CAR-T cell construction technology,especially in the development of chimeric antigen receptor component construction and gene transduction technology,and describes CAR-T cell products in line with the Good Manufactured Practices(GMP).Finally,the development,promotion and application of this technology are prospected. |
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| Keywords: | chimeric antigen receptor T cell immunotherapy chimeric antigen receptors construction gene transfer |
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