增生性瘢痕相关基因的筛选

目的　利用基因芯片技术寻找增生性瘢痕形成过程中的相关基因 ,并发现相关基因间的相互关系 ,探讨增生性瘢痕的机理。方法　选择 6例标本 ,3例增生性瘢痕与 3例正常皮肤 ,分成 3组 ,提取各个标本的总DNA ,制成荧光标记的cDNA探针 ,与含 4 0 0 0个人类靶基因的芯片 ( 15 0 0个为已知基因 ,2 5 0 0个为未报道或未知功能的基因 )杂交 ,经扫描、生物信息学分析比较两种组织间的差异表达。结果　增生性瘢痕与正常皮肤的差异表达基因在 3组间仅出现 1次的有 3 96个、重复 2次出现的有 186～ 2 4 2个、共同出现的有 116个 ,其中上调 10 8个、下调 8个 ,涉及 13大类基因 ,包括抑癌与原癌基因、细胞凋亡、细胞骨架与运动蛋白、细胞周期类蛋白等 ,有些为已知基因 ,有些仍未知其功能。结论　基因芯片扫描对研究增生性瘢痕有效可靠 ,发现多种基因参与了增生性瘢痕的形成过程

Study on differently expressed genes of hypertrophic scar and normal skin

Objective To screen the differently expressed genes between hypertrophic scar and normal skin by using cDNA chip and find out the relative genes and discuss on the mechanism of hypertrophic scar. Methods The total RNA from 3 hypertrophic scar cases and 3 normal skin were isolated from tissues and purified to mRNA by oligotex. They were reversely transcribed to cDNA with the incorporation of fluorescent dTUP to prepare the hybridization probes. Then, the mixed probes were hybridized to the 4?000 target genes cDNA chip (include 1?500 known genes and 2?500 EST) and scaned for the signals and found differences between scar and normal skin. Results There were 396 genes once and 116 genes thricd different significantly between hypertrophic scar and normal skin. Among 116 genes, there were 108 up-regulated and 8 down-regulated and included oncogene, apoptosis, cytoskeletion, protein of cell cycle. Conclusions It is effective to screen differently expressed genes between the two tissues by gene chip and a lot of genes are taking part in development of hypertrophic scar.