依达拉奉对大鼠急性脊髓损伤后Caspase-3、Bcl-xl表达影响

目的:研究依达拉奉对大鼠急性脊髓损伤后Caspase-3、Bcl-xl表达影响,探讨其对脊髓损伤后神经细胞的保护作用机制。方法:80只SD大鼠,随机分为假手术组(n=16),ASCI盐水组(n=32)和ASCI治疗组(n=32),采用改良的Allen法,制成中度脊髓损伤大鼠模型;各组按术后3h、6h、24h、48h随机分为4个亚组;检测各亚组脊髓组织MDA的含量;以免疫组化检测各亚组脊髓组织Caspase-3表达和Bcl-xl基因表达。结果:ASCI盐水组MDA含量、Caspase-3阳性细胞表达和Bcl-xl蛋白表达水平均明显高于假手术组;与盐水组相比,依达拉奉治疗组MDA含量和Caspase-3阳性细胞表达水平均下降,而Bcl-xl蛋白表达水平显著增高。结论:依达拉奉可抑制脊髓损伤后神经细胞凋亡,此作用可能与其减轻氧化激化、减弱Caspase-3表达和增强Bcl-xl表达水平有关。

Effect of edaravone on expressions of Caspase-3 and Bcl-xl after acute spinal cord injury in rats

Objective： To investigate the effects of edaravone on the expressions of Caspase-3 and Bcl-xl after acute spinal cord injury in rats. Method： A total of 80 health male SD rats were randomly divided into false-operation group （n= 16） and ASCI control group（n= 32） and ASCI treatment group（n= 32）. Each group was further divided into 4 subgroups, according to 3h, 6h, 24h and 48h after model esteblishment. The model of moderate spinal cord injury in rats was established by Alien method. The content of MDA was measured. The expressions of Caspase-3 and Bcl-xl were determined with immunohistochemistry. Results ： The content of MDA, the expression of Caspase-3 and Bcl-xl were significantly increased in the ASCI control group as compared with the false-operation group. Treatment of edaravone markedly reduced the content of MDA and the expression of Caspase-3 in comparison to the ASCI control group, while the expression of Bcl-xl was obviously higher in the ASCI treatment group than that in the ASCI control group. Conclusion： Edaravone inhibits the neuronal cell apoptosis after spinal cord injury, which might be related with the alleviated oxidative stress, decreased expression of Caspase-3 and increased expression of Bcl-xl.