超高效液相色谱-静电轨道阱质谱同时筛查测定猪肉中21种磺胺类药物残留

为保障居民饮食安全，加大水产品监管力度，提升检测效率，拟开发质谱高通量筛查水产品中药物残留的检测方法。

运用有机溶剂两步提取法提取水产品中的药物残留，采用C₁₈色谱柱分离、液相色谱-静电场轨道阱高分辨质谱筛查，并通过建立的质谱数据库定性。

建立195种兽药标准品质谱数据库和检测方法，开发了通过简单、快速提取水产品中兽药残留的前处理方法，检测方法灵敏度高、定性准确，80%以上化合物的加标回收率可达70%~110%。

本方法的建立能够实现水产品中兽药残留的高通量筛查，为食品安全风险监测和监管供技术支持，应对逐年增加的水产品需求和养殖量的检测需要，并且为后续的质谱高通量检测方法开发提供理论基础。

Simultaneous screening and determination of 21 sulfonamides residues in pork by ultra high-performance liquid chromatography electrostatic orbital trap mass spectrometry

In order to ensure the food safety of cultured aquatic products in China， strengthen the supervision and improve detection efficiency， a high-throughput screening method for drug residues in aquatic products by mass spectrometry is to be developed.Liquid chromatography-electrostatic field track well high resolution mass spectrometry and C₁₈ column were used to separate and collect the information of ion fragments and retention time of 195 veterinary drug standards in positive ion mode， and drug residues in aquatic products were extracted by organic solvents.The mass spectrum database and detection methods of 195 veterinary drug standards were established， and a pre-treatment method was developed for extracting veterinary drug residues from aquatic products by acetonitrile and ethyl acetate.
The detection method was sensitive， accurate， simple and rapid， and the adding standard recovery of more than 80% compounds reached 70%‒110%.The establishment of this method can quickly and massively screen drug residues in aquatic products， provide technical support for food safety risk monitoring and supervision， cope with the increasing demand for aquatic products and aquaculture volume year by year， and provide a theoretical basis for the subsequent development of mass spectrometry high-throughput detection methods.