6-OHDA诱导PD大鼠黑质神经细胞凋亡的观察

目的 探讨帕金森病发病过程中黑质神经元的缺失形式。方法 将6-OHDA立体定向微量注射于右侧内侧前脑柬(MFB)制备PD大鼠模型,观察大鼠的行为改变,并运用特殊染色,DNA原位末端标记技术检测黑质内多巴胺(DA)神经元凋亡的发生及其变化规律。结果 成功复制出符合临床特点的PD大鼠模型,且旋转行为与黑质区神经元数目呈反变关系。损伤侧黑质DA神经元存在着细胞凋亡。以术后两周最明显。结论 6-OHDA诱导的PD大鼠SNc中存在以凋亡为主的死亡方式。细胞凋亡在PD发病中可能起关键作用。

A Study of Apoptosis of Substantia Nigra Neurons in 6-OHDA-induced PD Model Rats

Objective To investigate the possible losing model of neurons in SNc during the development of Parkinson's disease(PD).Methods Using special-dyeing and TUNEL techniques,the changes of apoptosis,the course of Dopaminergic (DA) neurons in SNc and the change of behavior were observed after stereoscopically injecting 6-OHDA to right side of the medial forebrain bundle (MFB).Results The rotation rate in PD rat model was increased with the decrease of the DA neurons in SNc.Apoptosis was the main losing mode of DA neurons in PD.It was found that the high peak took place within 2 weeks after lesion.Conclusions The study showed that apoptotsis could be found in subtantia nigra neurons in 6-OHDA induced PD model rats,which may play a key role in the development of Parkinson's diseaes.