| 高效液相色谱法测定利奈唑胺滴眼液的含量 |
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| 引用本文： | 黄楚龙,王延东,曾夏芸,叶成添. 高效液相色谱法测定利奈唑胺滴眼液的含量[J]. 广东药学, 2012, 0(8): 464-466 |
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| 作者姓名： | 黄楚龙 王延东 曾夏芸 叶成添 |
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| 作者单位： | [1]中山大学中山眼科中心,广东广州510060 [2]广东宏盈科技有限公司,广东佛山528531 |
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| 基金项目： | 广东省部产学研合作引导项目（编号：2010B090400081）; 高明区科技三项费用项目（编号：201015） |
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| 摘 要： | 目的建立测定利奈唑胺滴眼液含量的高效液相色谱方法。方法采用Eclipse XDB-C8柱（4.6 mm×150 mm,5μm）为色谱柱;流动相：水∶乙腈（80∶20,v/v）,柱温50℃,流速1.4 ml/min,检测波长251 nm。结果在0.025～0.8 mg/ml浓度范围内,利奈唑胺峰面积和质量浓度呈良好的线性关系（r=0.999 5）,平均回收率为100.7%,RSD为0.6%,精密度高,耐用性好,样品溶液可以在室温环境下24 h内稳定。结论本方法专属性强,操作简便,结果准确,可用于测定利奈唑胺滴眼液的含量。
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| 关 键 词： | 高效液相色谱法 利奈唑胺 滴眼液 含量测定 |
Determination of Linezolid Eye Drops by HPLC |
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| HUANG Chu-long,WANG Yan-dong,ZENG Xia-yun,YE Cheng-tian. Determination of Linezolid Eye Drops by HPLC[J]. Guangdong Pharmaceutical Journal, 2012, 0(8): 464-466 |
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| Authors: | HUANG Chu-long WANG Yan-dong ZENG Xia-yun YE Cheng-tian |
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| Affiliation: | 1. Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China; 2. Guangdong Wholewin Technology. ,Ltd, Foshan, Guangdong 528531, China) |
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| Abstract: | Objective To establish an HPLC method for the determination of linezolid eye drops. Methods The separation was performed on Diamonsil C8 （150 mm ×4.6 mm, 5 μm） column, the mobile phase was water-acetonitrile （80： 20） with the flow rate of 1.4 ml/min, the detection avelength was at 251 nm and column temperature was 50℃. Results The calibration curve of linezolid was linear within the.oncentration range of 0. 025 -0.8 mg/ml, r = 0. 999 5. The average recovery rate of linezolid was 100.7% （RSI） = 0. 6% ）. Conclusion The method is simple, quick and specific. It can be used to determine the content of linezotid eye drops. |
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| Keywords: | HPLC linezolid eye drops determination |
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