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| 白藜芦醇增强TRAIL对人髓系白血病KG-1a细胞的细胞毒作用 | |
| 引用本文： | 胡亮杉,孙茂本,曾雅丽,李玉华,邓兰,郭坤元. 白藜芦醇增强TRAIL对人髓系白血病KG-1a细胞的细胞毒作用[J]. 中国免疫学杂志, 2011, 27(3): 217-219,227. DOI: 10.3969/j.issn.1000-484X.2011.03.006 |
| 作者姓名： | 胡亮杉 孙茂本 曾雅丽 李玉华 邓兰 郭坤元 |
| 作者单位： | 南方医科大学珠江医院血液内科,广州,510282 |
| 基金项目： | 国家自然科学基金(No.30973454)资助 |
| 摘 要： | 目的:观察白藜芦醇作用前后TRAIL对人髓系白血病KG-1a细胞的细胞毒作用的变化。方法:流式细胞仪检测KG-1a细胞表面CD34和CD38的表达,二甲氧唑黄(XTT)细胞增殖及细胞毒性检测试剂盒检测白藜芦醇作用前后TRAIL对KG-1a细胞增殖的影响,AnnexinV-FITC/PI染色流式细胞仪检测细胞凋亡变化。流式细胞仪检测白藜芦醇作用前后KG-1a细胞表面TRAIL死亡受体表达变化。结果:人髓系白血病KG-1a细胞CD34+CD38-占(58.67±2.87)%,10~1 000 ng/ml的TRAIL对KG-1a细胞增殖无明显影响,但对白藜芦醇作用后的KG-1a细胞的增殖有明显抑制作用,白藜芦醇能促进TRAIL诱导KG-1a细胞凋亡,并能上调KG-1a细胞表面TRAIL死亡受体DR5的表达。结论:白藜芦醇能增强TRAIL对人髓系白血病KG-1a细胞的细胞毒作用,其机制可能与白藜芦醇上调KG-1a细胞表面TRAIL死亡受体DR5的表达有关。 |
| 关 键 词： | 白藜芦醇 TRAIL 人髓系白血病KG-1a细胞 细胞毒作用 |
Resveratrol enhances cytotoxicity of TRAIL to human promyloblastic leukemia KG-1a cells |
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| HU Liang-Shan,SUN Mao-Ben,ZENG Ya-Li,LI Yu-Hua,DENG Lan,GUO Kun-Yuan. Resveratrol enhances cytotoxicity of TRAIL to human promyloblastic leukemia KG-1a cells[J]. Chinese Journal of Immunology, 2011, 27(3): 217-219,227. DOI: 10.3969/j.issn.1000-484X.2011.03.006 | |
| Authors: | HU Liang-Shan SUN Mao-Ben ZENG Ya-Li LI Yu-Hua DENG Lan GUO Kun-Yuan |
| Affiliation: | HU Liang-Shan,SUN Mao-Ben,ZENG Ya-Li,LI Yu-Hua,DENG Lan,GUO Kun-Yuan.Department of Hematology,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China |
| Abstract: | Objective:To explore the cytotoxicity of TRAIL to human acute promyeloblastic leukemia KG-1a cells treated with resveratrol.Methods:The expression of CD34 and CD38 on the surface of KG-1a cells was detected by flow cytometry;The effects of various concentration TRAIL on proliferation of KG-1a cells treated with or without resveratrol were analyzed by XTT cell proliferation and cytotoxicity assay kit;The changes of apoptosis in KG-1a cells treated with or without resveratrol induced by TRAIL were detected by... |
| Keywords: | Resveratrol TRAIL Human promyeloblastic leukemia KG-1a cells Cytotoxicity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |