| 聚乙二醇修饰的牛血红蛋白血液代用品抗原性和免疫原性的研究 |
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| 引用本文： | 侯继锋,叶苗.聚乙二醇修饰的牛血红蛋白血液代用品抗原性和免疫原性的研究[J].中国生化药物杂志,2005,26(1):29-31. |
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| 作者姓名： | 侯继锋 叶苗 |
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| 作者单位： | 1. 中国药品生物制品检定所,北京,100050
2. 浙江海康生物制品有限公司,浙江,温州,325000 |
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| 摘 要： | 目的建立聚乙二醇修饰的牛血红蛋白血液代用品抗原性和免疫原性评价方法。方法用酶联方法测定供试品的抗原性残留量,用豚鼠过敏试验研究供试品的免疫原性,通过比较推算供试品抗原性残留量的安全性限值。结果供试品的抗原性残留量大于2 3%时,豚鼠会出现不同程度的过敏反应;供试品的抗原性残留量小于或等于2 3%时,豚鼠无过敏反应发生;供试品抗原性残留量的最高限值为2 3%。结论初步建立了牛血红蛋白血液代用品抗原性和免疫原性评价方法
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| 关 键 词： | 血液代用品 抗原性 免疫原性 |
| 文章编号： | 1005-1678(2005)01-0029-03 |
| 修稿时间： | 2004年2月16日 |
Study on antigenicity and immunogenicity of PEG modified bovine hemoglobin for blood substitute |
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| HOU Ji-feng,YE Miao.Study on antigenicity and immunogenicity of PEG modified bovine hemoglobin for blood substitute[J].Chinese Journal of Biochemical Pharmaceutics,2005,26(1):29-31. |
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| Authors: | HOU Ji-feng YE Miao |
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| Institution: | HOU Ji-feng~1,YE Miao~2 |
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| Abstract: | PurposeTo establish methods used for evaluating antigenicity and immunogenicity of PEG modified bovine hemoglobin for blood substitute.MethodsAntigenicity residue and immunogenicity of samples were tested by ELISA and guinea pig hypersensitive test respectively. Antigenicity residue limit was defined by comparing these two results.ResultsIf antigenicity residue was more than 23%,guineas pig would have hyper-sensitive reaction. Guineas pig would have no hypersensitive reaction when antigenicity residue was no more than 23%. Antigenicity residue limit for samples should be no more than 23%. ConclusionMethods were primarily established and used for evaluating antigenicity and immunogenicity of PEG modified bovine hemoglobin for blood substitute. |
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| Keywords: | blood Substitute antigenicity immunogenicity |
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