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| 多色荧光原位杂交及其在遗传毒理学中的应用 | |
| 引用本文： | 夏彦恺,王心如. 多色荧光原位杂交及其在遗传毒理学中的应用[J]. 卫生研究, 2004, 33(3): 354-357 |
| 作者姓名： | 夏彦恺 王心如 |
| 作者单位： | 南京医科大学应用毒理学研究所,南京,210029 |
| 基金项目： | 国家基础研究重大项目前期研究专项基金 (No.2 0 0 1CCA0 4 90 0 ) |
| 摘 要： | 多色荧光原位杂交 (MFISH)在检测核酸序列、染色体和基因方面有着强大功能。由于染色体结构和数目畸变被认为是遗传毒性研究重要的生物学终点 ,而该方法可准确直观地评价暴露于职业、医疗和环境毒物后的染色体异常 ,因此以其为基础的各类方法在人类遗传学、生殖医学、尤其是遗传毒理学中将会得到广泛的应用。 |
| 关 键 词： | 多色荧光原位杂交 染色体畸变 非整倍体 遗传毒理学 |
| 文章编号： | 1000-8020(2004)03-0354-03 |
| 修稿时间： | 2003-09-25 |
Multicolor fluorescence in situ hybridization and its application in genetic toxicology |
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| Xia Yankai,Wang Xinru Institute of Applied Toxicology,Nanjing Medical University,Nanjing ,China. Multicolor fluorescence in situ hybridization and its application in genetic toxicology[J]. Journal of hygiene research, 2004, 33(3): 354-357 | |
| Authors: | Xia Yankai Wang Xinru Institute of Applied Toxicology Nanjing Medical University Nanjing China |
| Affiliation: | Institute of Applied Toxicology, Nanjing Medical University, Nanjing 210029, China. |
| Abstract: | Multicolor fluorescence in situ hybridization, a new promising technology today, offers unparalleled capabilities for detection of nucleic acid sequences, chromosomes and genes. Owing to structural and numerical chromosomal aberrations were considered as important biological end points in genotoxic studies, chromosomal aberrations after exposure to occupational, medical, and environmental toxicants could be accurately and appropriately evaluated via this assay. Consequently, multicolor FISH-based methods could be widespread applied in human genetics, reproductive medicine, especially genetic toxicology. In this paper, the progress in regard was reviewed. |
| Keywords: | multicolor fluorescence in situ hybridization(MFISH) chromosomal aberrations aneuploidy genetic toxicology |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |