葛根素注射液对自发性高血压大鼠AT1和ACE2 mRNA表达的影响

目的 探讨葛根素对自发性高血压大鼠Ⅰ型血管紧张素Ⅱ受体（AT1）及血管紧张素转换酶2（ACE2）的作用。 方法 将12周龄自发性高血压大鼠分成4组：模型对照组、维拉帕米组、低剂量葛根素组及高剂量葛根素组，给药3周后，取心肌、主动脉、肾脏组织提RNA。采用RT-PCR技术检测AT1和ACE2 的mRNA表达水平。 目的 探讨葛根素对自发性高血压大鼠Ⅰ型血管紧张素Ⅱ受体（AT1）及血管紧张素转换酶2（ACE2）的作用。 目的 探讨葛根素对自发性高血压大鼠Ⅰ型血管紧张素Ⅱ受体（AT1）及血管紧张素转换酶2（ACE2）的作用。

Effect of Puerarin Injection on the mRNA Expressions of AT1 and ACE2 in Spontaneous Hypertension Rats

Objective To study the effect of puerarin on angiotensin Ⅱ type 1 receptor （AT1） and angiotensin-converting enzyme 2 （ACE2） in spontaneous hypertension rat （SHR）. Methods SHRs, 12 weeks old, were randomly divided into four groups： the model control group （A） , the Verapamil group （B） , and the two puerarin groups （ C and D） treated by low dose and high dose of puerarin respectively. After being treated for 3 weeks, total RNA from tissues of heart, aorta and kidney in rats were extracted and mRNA expression levels of AT1 and ACE2 were determined by RT-PCR. Results As compared with Group A, the mRNA expressions of AT1 and ACE2 in heart tissue were lower in Group C, and those in kidney tissue were higher in Group D （ all P 〈 0.05） ; ACE2 mRNA expression was higher in Group D than in Group C （ P 〈 0.05 ） ; no significant differences of the two indexes in aorta were shown among various groups. Besides, mRNA expressions of AT1 and ACE2 in heart and kidney tissue were proved to be positively linearly correlated. Conclusion High dose puerarin could increase the mRNA expressions of AT1 and ACE2 in kidney, while low dose puerarin could decrease them in heart; there might be a feed back correlation between AT1 and ACE2.