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| 高侵袭转移Lovo细胞亚系的建立及其生物学特性的研究 | |
| 引用本文： | 陈艾保,丁彦青,谢小棉,刘炽,张煜.高侵袭转移Lovo细胞亚系的建立及其生物学特性的研究[J].肿瘤研究与临床,2002,14(2):85-88. |
| 作者姓名： | 陈艾保 丁彦青 谢小棉 刘炽 张煜 |
| 作者单位： | 1. 解放军第157中心医院，广东 广州 510510 2. 第一军医大学肿瘤研究所 广东 广州 510510 3. 第一军医大学，广东 广州 510510 4. 湖南省中医学院附二院，湖南 长沙 410000 |
| 摘 要： | 目的 :建立高侵袭 L ovo细胞亚系以及研究其生物学特性。方法 :应用裸鼠体内连续传代法 ,筛选并建立高侵袭 L ovo细胞亚系 ,并测量 L ovo亚系电泳泳动速率、细胞周期和 DNA含量。结果 :L ovo亚系细胞电泳泳动速率明显高于其母系 L ovo细胞 ;其增殖周期 (S G2 M)百分率为 5 7.4 % ,高于其母系 L ovo细胞的 4 1.7% ,且其 DNA含量也远高于其母系细胞。结论 :L ovo亚系细胞比 L ovo母系细胞具有更高侵袭力和更快的增殖能力 |
| 关 键 词： | Lovo细胞 亚系 电泳 DNA含量 |
| 文章编号： | 1006-9801(2002)02-0085-04 |
| 修稿时间： | 2001年9月7日 |
CONSTRUCTING A HIGH METASTASIS SUBLINE FROM LOVO CELL AND STUDYING ITS BIOLOGICAL CHARACTERISTICS |
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| CHEN Ai bao,DING Yan qing,XIE Xiao mian,et al..CONSTRUCTING A HIGH METASTASIS SUBLINE FROM LOVO CELL AND STUDYING ITS BIOLOGICAL CHARACTERISTICS[J].Cancer Research and Clinic,2002,14(2):85-88. | |
| Authors: | CHEN Ai bao DING Yan qing XIE Xiao mian |
| Institution: | CHEN Ai bao,DING Yan qing,XIE Xiao mian,et al.The 157 central Hospital of PLA |
| Abstract: | To construct a strong invasive lovo cell subline and to study its metastasis.Methods:To apply a continuous go down to the future generation method of mouse,selecting and constructing strong invasive lovo cell subline from spleen inoculation through repeated experimental metastasis.To examine lovo cell subline electrophoresis migration rate and it's period and content of DNA. Result:The electrophoresis migration rate of lovo cell subline was obviously higher than that of its mother lovo cell;it's cycline(S G 2M)% was 57.4 % and that of lovo cell was 41.7 %,but content of DNA of lovo cell subline was higher than that of lovo cell.Conclusions:This research suggested that lovo cell had stronger subline metastasis and rapid proliferation than that of its mother line lovo cell. |
| Keywords: | Lovo cell Subline Electrophoresis migration Content of DNA |
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