| Ad-p53与Ad-p16联合对人肺腺癌GLC-82细胞作用的体外实验研究 * |
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| 引用本文： | 徐敏毅,林 晨,梁 萧,付 明,张雪艳,吴旻.Ad-p53与Ad-p16联合对人肺腺癌GLC-82细胞作用的体外实验研究 *[J].中国肿瘤生物治疗杂志,2000,7(2):86-89. |
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| 作者姓名： | 徐敏毅 林 晨 梁 萧 付 明 张雪艳 吴旻 |
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| 基金项目： | * 本课题受863计划项目(Z20-01-02)资助 |
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| 摘 要： | 探讨腺病毒介导的p53与p16基因的联合对人肺腺病GLC-82疗效提高的可能性。方法：将分别携有p53基因、p16基因重组腺病毒(Ad-p53与Ad-p16)联合使用，通过细胞生长和存活实验、克隆形成实验、流式细胞分析、TUNEL检测、RT-PCR分析、免疫组织化学实验，观察其对人肺腺癌GLC-82细胞的作用。结果：在总感染强度相同的前提下，Ad-p53与Ad-p16联合导入GLC-82细胞，对细胞生长存活及克隆形成能力的抑制、以及所造成的凋亡效果比施用单种基因的效果强，表明p53基因与p16基因在体外疗效上互为协同。结论： Ad-p53与Ad-p16联合，可以提高对人肺腺癌GLC-82细胞的疗效。
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| 关 键 词： | 肿瘤基因治疗 联合治疗 |
| 收稿时间： | 1999/8/11 0:00:00 |
| 修稿时间： | 1999/11/29 0:00:00 |
The Experimental Studies of Ad-p53 and Ad-p16 in Combination on Human Lung Adenocarcinoma Cell Line GLC-82 |
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| Xu Minyi,Lin Chen,Liang Xiao,Fu Ming,Zhang Xueyan and Wu Min.The Experimental Studies of Ad-p53 and Ad-p16 in Combination on Human Lung Adenocarcinoma Cell Line GLC-82[J].Chinese Journal of Cancer Biotherapy,2000,7(2):86-89. |
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| Authors: | Xu Minyi Lin Chen Liang Xiao Fu Ming Zhang Xueyan and Wu Min |
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| Institution: | National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021;National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021;National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021;National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021;National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021;National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021 |
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| Abstract: | To determine the effect of Ad-53 and Ad-16both alone and in combination on the growth of human lung adeuocarcinoma cell line GLC-82. Methods: Human lung adenocarcinoma cell line GLC-82 were trasferredvia an adenovirus-mediated p53 gene (Ad-p53), P16 gene Ad-p16 or both. Cell growth inhibition assay, colony formation, flow cytometry assay, TUNEL, RT-PCR and immunhistochemistry assays were used to evaluate the therapeutic efficiency of such strategy on trasferred cells. Results: in vitro experiments, at the same dose ofAd (multiplicity of infection), simultaneous transfer of Ad-p53 and Ad-p16 to GLC-82 cells caused stronger therapeutic efficacy than caused by single kind of Ad transfer, demonstrating that Ad-p53 treatment has synergistic efficacy with Ad-p16 treatment on GLC-82 cells. Conclusion: Ad-p53 for GLC-82 cells shows enhanced therapeutic efficiency when combined with Ad-p16. |
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| Keywords: | cancer gene therapy combined therapy |
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