Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5) |
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| Authors: | J. von Overbeck C. Stähli F. Gudat H. Carmann C. Lautenschlager U. Dürmüller B. Takacs V. Miggiano Th. Staehelin Ph. U. Heitz |
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| Affiliation: | (1) Department of Pathology, University of Basel, P.O. Box CH-4003, Basel;(2) Central Research Department, F. Hoffmann-La Roche Co, Basel, Switzerland |
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| Abstract: | ![]()
Summary A mouse monoclonal antibody (mAB lu-5) was prepared using a lung cancer cell line as an antigen. The selected clone produces an IgG with a gamma-1 heavy chain and a kappa-light-chain. Immunohistochemical testing of mAB lu-5 on 117 normal tissue biopsies and 474 tumours revealed reactivity with an intracytoplasmic, formaldehyderesistant antigen present in most epithelial and mesothelial cells, but absent in mesenchymal cells. The antibody can therefore be used as a first order, pan-epithelial marker. It proved also useful for fast tumour diagnosis on frozen sections. |
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| Keywords: | Monoclonal pan-epithelial antibody Immunohistochemistry Tumour diagnosis |
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