组织因子及其抑制剂-1、2与肿瘤转移及血栓形成的相关性研究

 目的　探讨组织因子（TF）、组织因子抑制剂（TFPI）-1、2在肿瘤转移和血栓形成中的作用。方法　收集292例恶性肿瘤患者血液和组织标本，以130位门诊体检人员作对照组。ELISA法检测血浆TF、TFPI-1和TFPI-2浓度；免疫组织化学染色观察TF、TFPI-1和TFPI-2的表达。结果　各肿瘤组TF以及TFPI-1血浆浓度均高于对照组（P＜0.05），肺癌组TF和TFPI-1血浆浓度分别为（3.34±1.12）U／L、（156.13±57.12）μg／L，高于其他类型肿瘤组；各组TFPI-2差异无统计学意义（P＞0.05）。各肿瘤组TF组织表达均较瘤旁组织增高（P < 0.05）；TF在肺癌组织表达显著高于其他肿瘤组织（P < 0.05）。肿瘤合并血栓形成组与无血栓形成组TF、TFPI-1血浆浓度均较对照组增高（P < 0.05），TF在血栓形成组较无血栓形成组明显增高（P < 0.05）。结论　多种肿瘤高表达TF，不同肿瘤类型表达部位不同，类型不同的肿瘤表达强度不尽相同。血循环中TFPI-1和TF的失衡状态可能与肿瘤的高凝状态有关，促进了肿瘤血栓形成。

Research about the relationship of tissue factor, tissue factor pathway inhibitor-1, 2 with cancer metastases and thrombosis

Objective To evaluate the effect of tissue factor (TF), tissue factor pathway inhibitor-1,2 (TFPI-1,2) on cancer metastases and thrombosis complicated with cancer. Methods Blood samples from 292 cancer patients were collected and were divided into different teams according to cancer types,complicated with or without thrombosis; TF, TFPI-1, TFPI-2, plasma concentrations were measured by ELISA;Tissue expression of TF, TFPI-1, TFPI-2 were observed by immunohistochemical method. Results Plasma concentrations of TF and TFPI-1 in all kinds of cancers were higher than the control and lung cancer was the highest; TFPI-2 plasma concentrations had no statistics differences among all these teams. Tissue expression of TF of all kinds of cancer were higher than the adjacent tissues, lung cancer was higher than the other types of cancer. There were no statistics differences for TFPI-1 and TFPI-2. Both TF and TFPI-1 plasma concentrations of cancer with-or without-thrombosis were higher than control. TF was even higher in cancer with thrombosis team, TFPI-1 had no statistic difference between these two teams. TFPI-2 concentrations had no differences among all these teams. Conclusion Many kinds of tumor have higher expression of TF, it is expressed with different intensity according to different types of cancer. TFPI-1 has no clear effect in cancer growing and metastases. Unbalance of TF and TFPI-1 in plasma may relate to high coagulation state of cancer and may accelerate the thrombosis formation in cancer.