适于杂交瘤细胞长期大量繁殖的SCI－8910培基的研制

单抗的免疫导向治疗已成为国内外肿瘤研究的热点之一。目前杂交瘤分泌单抗的来源一部分是小鼠腹水，另一部分培养于含血清的培养基内，这对单抗纯化带来困难，对免疫导向治疗也带来许多不利条件，如牛的ＩｇＧ、鼠的病毒等的污染。因此，我们着手研制一种能适应于杂交瘤长期培养的无血清培基，定名为ＳＣＩ－８９１０。现已进行了９株杂交瘤细胞的长期培养，同时用该培基在１．５Ｌ的Ｃｅｌｌｉｇｅｒ（ＮＢＳＵＳＡ）搅拌生物反应器中长期培养鼠－鼠杂交瘤细胞２Ｆ７（分泌抗人小细胞肺癌单抗）获得成功，经检测单抗纯度达到一定要求，产品在８ｍｇ／Ｌ以上。结果提示，该无血清培基制备的ＭＣＡｂ用于免疫导向治疗的可行性及大规模生产的可能性。

Studies on Long-term Growth of Several Hybridoma Cell Lines in SCI-8910 Serum-free Medium

Production and purification of monoclonal antibodies are necessary for clinical application.A serum-free medium that can support the long-term growth and largescale propagation of hybridoma cells is a necessity.We have developed a new serumfree medium designated SCI-8910,which supports long-term growth of nine Sp2/0derived hybridoma cell lines secreting monoclonal antibodies against human small cell lung cancer(2F7,5E2,116),human hepatoma(LH-2F7),human transferrin(2C5),Fibri protein(2H4), human colonic tumor(8E9,7B10) and human CEA antigen.The sources of materials used in preparation of SCI-8910 medium were as follow:Dulbecco's Eagle'S minimal essential medium(DMEM),F12 medium supplemented with insulin,human transferrin,slenium and other,pH7.0-7.2.The vitality of hybridoma cells in SCI-8910 was similar to or higher than that in serum-supplemented DMEM medium.By using Western blot analysis it is confirmed that the monoclonal antibody concentrated from the Supernatant of 5E2 is specific for H128 antigen.