内皮素和一氧化氮与老年动脉硬化闭塞症相互关系的探讨

目的 从分子生物学角度探讨内皮素（ＥＴ）、一氧化氮（ＮＯ）与动脉硬化闭塞症（ＡＳＯ）的相互关系。方法 选择９６例ＡＳＯ患者和８６只模型，利用放射免疫、Ｇｒｉｓｓ、光电比色和逆转录ＰＣＲ方法检测血浆ＥＴ、ＮＯ和血管一氧化氮合酶（ＮＯＳ）活性、ＥＴｍＲＮＡ和ＮＯＳｍＲＮＡ表达情况。结果 ＡＳＯ患者和模型均存在高ＥＴ血症和低ＮＯ血症，ＡＳＯ病变血管存在低ＮＯＳ活性、低ＮＯＳｍＲＮＡ表达和高ＥＴｍＲＮＡ表

The study of relationship between endothelins, nitric oxide and atherosclerosis obliterans

Objective To investigate the relationship betwwen endothelins(ET),nitric oxide(NO),and atherosclerosis obliterans(ASO)from the point of view of molecular biology.Methods 96 ASO patients and 86 ASOrabbit models were selected or made.ET,NO,NOS activity,and ET-mRNA,NOS-mRNA expression were determinde by radioimmunoassy,colorimetry and RT-PCR methods in the plasma samples and ASO arterial samples.Results The ASO patients and models had high levels of plasma ET and low level of plasma NO which were related to severity of ASO,and the ETmRNA expressions of arterial samples were increased gradually,the NOSmRNA expressions and the NOS activity of arterial samples were decreased gradually with the time of ASO model making process.All changes above returned to normal gradually with effective PGEI1 treatment.Conclusion The abnormalitis of ET,NO,NOS activity,ETmRNA,NOSmRNA expressions are closely related to ASO severity and play the important role in the ASO pathogenesis.It is helpful to judge the ASO serverity and to evaluate the effectiveness of ASO treatments.