阿托伐他汀钙预防大鼠肝缺血再灌注损伤的实验研究

目的探讨阿托伐他汀钙对肝缺血再灌注损伤的保护作用及其作用机制。方法采用在体大鼠肝原位缺血再灌注模型。21只健康雄性SD大鼠随机分成假手术组(N)、缺血再灌注组(I/R)、阿托伐他汀钙处理组(AT+I/R),每组7只。各组于再灌注90 min后经肝上下腔静脉取血,用于检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST);取肝匀浆低温离心后采用试剂盒测定肝组织中总超氧化物歧化酶(TSOD)、丙二醛(MDA)、髓过氧化物酶(MPO)、一氧化氮(NO)、一氧化氮合酶(NOS)含量变化;同时取肝脏组织作病理切片观察。结果 AT+I/R组较I/R组:ALT分别为(928.43±96.17)、(1234.89±327.17)IU/L;AST分别为(1 222.91±249.85)、(1635.74±481.86)IU/L;MDA分别为(2.35±0.57)、(3.29±1.23)nmol/mg;MPO分别为(2.33±0.71)、(3.18±0.82)IU/mg;NO分别为(89.27±8.27)、(102.32±11.81)μmol/mg;iINOS分别为(0.25±0.14)、(0.45±0.21)U/mg,AT+I/R组明显降低(P<0.05),而SOD(20.86±2.49)、(14.58±2.04)IU/mg;cNOS(0.69±0.17)、(0.56±0.13)U/mg明显升高(P<0.05)。结论阿托伐他汀钙有保护肝缺血再灌注损伤的作用。其可能机制为激活了抗氧自由基、提高机体清除氧自由基、抗炎作用和减轻钙超载有关。

The effects of Atorvastatin on liver ischemia-reperfusion injury in rats

Objective To explore the protective effect of atorvastatin on liver and its possible mechanism during the liver ischemia-reperfusion injury.Methods Single liver in site ischemia-reperfusion rats model was used.Twenty-one healthy male SD rats were randomly divided into three groups,each consisting of 7 rats: sham operate group(N),ischemia-reperfusion group(I/R) and atorvastation treatment group(AT+I/R).After 90 minutes′ reperfusion,blood was collected in each group by the superior and inferior vena cava of liver so as to detect alanine arninotransferase(ALT) and asparate aminotransferase(AST).Liver homogenate was centrifuged at low temperature,and then the content variation of superoxide dismustase(SOD),malondialdehyde(MDA),myeloPeroxidase(MPO),nitric oxide(NO) and nitrieoxidesynthas(NOS) in liver tissue were measured by kit.Meanwhile,liver tissue was observed by light microscope.Results Compared group AT+I/R with group I/R: ALT [(928.43±96.17) vs.(1 234.89±327.17)] IU/L;AST [(1 222.91±249.85) vs(1 635.74±481.86)] IU/L;MDA [(2.35±0.57) vs.(3.29±1.23)] nmol/mg;MPO [(2.33±0.71) vs.(3.18±0.82)] IU/mg;NO [(89.27±8.27) vs.(102.32±11.81)] μmol/mg;iINOS [(0.25±0.14) vs.(0.45±0.21)] U/mg decreased(P<0.05) and SOD [(20.86±2.49) vs(14.58±2.04)] IU/mg;cNOS [(0.69±0.17) vs.(0.56±0.13)] U/mg increased(P<0.05).Conclusion Atorvastatin could protect liver from ischemia-reperfusion injury.Its possible mechanisms are activating antioxidant free radicals and improving the effects of organisms,that is,scavenging oxygen free radicals and anti-inflammatory.