虎杖对肺纤维化大鼠肺组织中SOD、GSH、MDA的影响

目的：通过观察虎杖干预后肺纤维化大鼠肺组织中超氧化物歧化酶（SOD）活性及谷胱甘肽（GSH）、丙二醛（MDA）含量的影响，探讨虎杖防治肺纤维化的作用机制。方法健康雄性SD大鼠120只，随机分为5组：模型组30只，气管内一次性注入博莱霉素50.5mg/100g制作肺纤维化大鼠模型，当日予0.9%氯化钠溶液5ml/100g灌胃；预防组30只，于造模前2d开始予虎杖5ml/100g灌胃；肺泡炎组20只，于造模后第7天开始虎杖灌胃；肺纤维化组10只，于造模后第28天开始虎杖灌胃；对照组30只，于造模当日开始0.9%氯化钠溶液5ml/100g灌胃。各组大鼠灌胃均1次/d。5组大鼠分别于第3、7、14、28、42或56天分阶段处死5只，取肺组织制成匀浆，测定SOD活性及GSH、MDA的含量。结果模型组第3、7、14天肺组织中SOD活性及GSH含量低于对照组（均P＜0.05），预防组上述指标较模型组升高（P＜0.05或0.01），肺泡炎组、肺纤维化组与模型组的差异均无统计学意义（均P＞0.05）。模型组第7、14天肺组织中MDA含量明显高于对照组和预防组（均P＜0.05）。结论虎杖能通过增强肺组织局部抗氧化能力，减轻博莱霉素诱导的肺泡炎和肺纤维化程度，可能是治疗肺纤维化的有效药物。

Effect of Rhizoma Polygoni Cuspidati on SOD,GSH and MDA contents in lung tissue of rats with bleomycetin-induced pulmonary fi- brosis

Objective To evaluate the effects Rhizoma Polygoni Cuspidati on SOD,GSH and MDA contents in lung tissue of rats with bleomycetin- induced pulmonary fibrosis. Methods One hundred and twenty healthy male SD rats were randomly divided in to 5 groups. Pulmonary fibrosis model was induced by intratracheal infusion of 50.5mg/100g bleomycetin. Rats in model group （n=30） were instil ed with saline 5ml/d by gavage after bleomycetin was infused;rats in preventive group（n=30）were treated with liquid extract of Rhizoma Polygoni Cuspidati 2d before bleomycetin infused;rats in alveolitis group （n=20）and pul-monary fibrosis group （n=10） were treated with liquid extract of Rhizoma Polygoni Cuspidati 7d after bleomycetin infused;rats in control group （n=30） were instil ed with saline 5ml/d. Five rats in each group were sacrificed on d3,7,14,28,42 and 56, and lung samples were col ected. The contents of SOD,GSH and MDA in lung homogenate were assayed. Results The levels of SOD and GSH on d 3,7 and 14 were lower in model group than those in control group（P〈0.05） and preventive group（P〈0.05）;the levels of MDA on d7 and 14 were higher in model group than those in control group and preventive group （P〈0.05）. Conclusion Liquid extract of Rhizoma Polygoni Cuspidati has anti- oxidant effects, which is associated with the al eviation of alveolitis pulmonary fi-brosis induced by bleomycetin in rats.