| 高效液相色谱法测定野菊花中木犀草素的含量 |
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| 引用本文： | 钱频非,葛滨,王殿广. 高效液相色谱法测定野菊花中木犀草素的含量[J]. 中国药房, 2005, 16(22): 1741-1742 |
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| 作者姓名： | 钱频非 葛滨 王殿广 |
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| 作者单位： | 1. 江苏东南大学医院,南京市,210018
2. 南京生物工程与医药科技发展有限公司,南京市,210029 |
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| 摘 要： | 目的:建立以高效液相色谱法测定野菊花中木犀草素含量的方法。方法:色谱柱为Kromasil C18,流动相为四氢呋喃-甲醇-1.5%醋酸(27∶4∶69),流速为1.0ml/min,检测波长为353nm,柱温为30℃。结果:木犀草素进样量在0.028μg~0.56μg范围内线性关系良好(r=1.0 000),平均回收率为99.9%(RSD=2.28%)。结论:本方法操作简便,结果可靠,可用于野菊花药材的质量控制。
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| 关 键 词： | 高效液相色谱法 野菊花 木犀草素 含量测定 |
| 文章编号： | 1001-0408（2005）22-1741-02 |
| 收稿时间： | 2004-11-22 |
| 修稿时间： | 2005-01-28 |
Content Determination of Luteolin in Wild Chrysanthemum Flower by HPLC |
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| QIAN Pinfei,GE Bin,WANG Dianguang. Content Determination of Luteolin in Wild Chrysanthemum Flower by HPLC[J]. China Pharmacy, 2005, 16(22): 1741-1742 |
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| Authors: | QIAN Pinfei GE Bin WANG Dianguang |
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| Affiliation: | 1.The Affiliated Hospital of Southeast University in Jiangsu Province,Nanjing 210018,China;2. Nanjing Bioengineering and Medicine Technology Development Co.Ltd., Nanj ing 210029, China |
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| Abstract: | OBJECTIVE:To establish a HPLC method for content determination of luteolin wild chrysanthemum flower.METHODS:The separation was performed on a Kromasil C18 column,the mobile phase was composed of tetrahydrofuran-methanol-1.5%acetic acid(27∶4∶69),the flow rate was 1.0 ml/min,the ultraviolet wavelength was 353nm,the column temperature was 30℃.RESULTS:The calibration curve showed good linearity in the range of 0.028μg～0.56μg(r=1.0 000),the average recovery was 99.9%(RSD=2.28%).CONCLUSION:The method is simple,convenient and accurate and can be used for the quality control of wild chrysanthemum flower. |
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| Keywords: | HPLC Wild chrysanthemum flower Luteolin Content determination |
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