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Ki-ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N-Nitrosobis(2-hydroxypropyl)amine |
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| Authors: | Toshikazu Ushijima Masahiro Tsutsumi Ryuichi Sakai Yukihito Ishizaka Fumimaro Takaku Yoichi Konishi Michihito Takahashi Takashi Sugimura Minako Nagao |
| Affiliation: | Carcinogenesis Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104;Department of Oncological Pathology, Cancer Center, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634;The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113;Division of Pathology, National Institute of Hygienic Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158 |
| Abstract: | Five pancreatic carcinomas induced by N -nitrosobis(2-hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki- ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki- ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G-to-A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N -nitrosobis(2-oxopropyl)amine. |
| Keywords: | Ki-ras Hamster Pancreatic carcinoma N-Nitrosobis(2-hydroxypropyl) amine |