利用组织芯片探讨PTEN在喉鳞状细胞癌中的表达

目的:探讨PTEN在喉癌中的表达以及与病理学分级、临床TNM分期和预后等因素的关系。方法:应用组织芯片技术结合免疫组织化学、原位杂交的方法对146例喉鳞状细胞癌、40例癌前期病变以及26例声带息肉患者组织中PTENmRNA以及蛋白的表达,结合临床相关因素进行统计分析。结果:PTEN蛋白在声带息肉中无表达,癌前期病变以及喉癌组织的阳性表达率分别为40.00%、43.15%。在喉癌组织中的表达与喉癌的病理分级以及预后差异有统计学意义,与发生部位、临床分期、淋巴结转移差异无统计学意义。PTEN在癌前期病变及喉癌组织的原位杂交阳性表达率分别为77.50%与59.59%。PTEN在癌前期病变以及喉癌组织的原位杂交阳性表达率高于免疫组织化学的蛋白阳性表达率。结论:组织微阵列是一种高效率的实验方法,具有快速、高精确度、节约资源的特点。PTEN的表达与喉癌的预后有关。

Research of the expression of PTEN in squamous cell carcinoma of larynx by the tissuechip technology

Objective:To evaluate the expression of PTEN in squamous cell carcinoma of larynx and its relationship with factors like pathologic fractionation,clinical TNM stage,and prognosis by the tissuechip technology.Method:We studied the expression of PTEN gene and its mRNA on a series of 146 cases of primary laryngeal carcinoma patients,40 cases of precancerous lesion and 26 cases of vocal fold polyp by tissuechip by,immunohistochemistry and in situ hybridization method.The observed data observed and some relevant clinical dada were statistically analyzed.Result:The expression of PTEN in vocal fold polyp was negative,and its positive expression in precancerous lesion and laryngeal carcinoma were 40% and 43.15% respectively by immunohistochemistry,and were 72.50%and 59.59% respetively in situ hybridization.The difference between the expression of PTEN in laryngeal carcinoma and its pathological fraction and prognosis was statistically significant,but was not significant between that and location,clinical stage and LN metastasis.The mRNA expression of PTEN was higher than that of the protein expression in precancerous lesion and cancer tissue.Conclusion:The tissue microarray technique required shorter time and less expense,and showed higher consistency in our essays.And the present study suggests PTEN was a prognosis factor of the Laryngeal carcinoma.