5-磷酸二酯酶抑制剂对庆大霉素所致豚鼠耳毒性影响的研究

目的 探讨5-磷酸二酯酶(PDE5)抑制剂对庆大霉素所致豚鼠耳毒性的听功能及耳蜗形态学的影响.方法 采用随机数字表法将36只豚鼠分为空白对照组、庆大霉素组和PDE5抑制剂组,每组各12只;其中空白对照组未给予特殊处理,庆大霉素组和PDE5抑制剂组给予连续腹腔注射庆大霉素120 mg·kg-1·d-13周后,庆大霉素组继...

The Experimental Study on the Effect of the Inhibitor of PDE5 on the Ototoxicity of Gentamicin in Guinea Pigs

Objective To study the effects of the inhibitor of PDE5 on the hearing function and cochlea morphology of guinea pigs to the ototoxicity of gentamicin.Methods A total of 36 guinea pigs were randomly divided into control group,gentamicin control group and the inhibitor of PDE5 group,12 in each group.Three weeks after gentamicin 120 mg·kg^-1·d-1 injected into guinea pigs of the inhibitor of PDE5 group and gentamicin control group,tadalafil(2 mg·kg^-1·d-1)and NS(4 ml·kg^-1·d-1)were injected into guinea pigs of above two groups respectively.ABR thresholds and the PL of waveⅠwere measured respectively prior to the experiment,1,2 and 4 weeks post-drugs,the changes of cochlea hair cells were also observed by a scan electron microscope(SEM).Results The ABR threshold shifts and the PL of waveⅠin the inhibitor of PDE5 group were significantly fewer than that in the gentamicin control group after the administration.SEM showed that the outer hair cells in gentamicin control group displayed mess,fusion and imperfections.In the inhibitor of PDE5 group,the hair cells displayed slight pathological changes,there were no significant differences compared with normal group.Conclusion The inhibitor of PDE5 can significantly protect against the ototoxicity of gentamicin.