丹参和三七有效组分对氧糖剥夺损伤的大鼠星形胶质细胞谷氨酸摄取的影响

目的探讨丹参、三七有效组分单独及联合使用对氧糖剥夺/复氧(OGD/R)诱导的星形胶质细胞谷氨酸摄取能力的影响。方法体外培养原代Wistar大鼠大脑皮层星形胶质细胞,分为8个组别:正常组,模型组,低、高剂量丹参多酚酸组,低、高剂量血栓通组和低、高剂量联合组。正常组和模型组均不给药;低、高剂量丹参多酚酸组分别给予12.5和25.0μg·mL^-1丹参多酚酸;低、高剂量血栓通组分别给予3.125和6.25μg·mL^-1血栓通;低、高剂量联合组分别给予12.5μg·mL^-1丹参多酚酸联合3.125μg·mL^-1血栓通,25.0μg·mL^-1丹参多酚酸联合6.25μg·mL^-1血栓通。正常组给予常规培养;模型组进行OGD 3 h/R 3 h的处理;给药组于给药2 d后进行OGD 3 h/R 3 h的处理。用酶标仪检测细胞活力和谷氨酸含量。结果正常组,模型组,低、高剂量丹参多酚酸组,低、高剂量血栓通组和低、高剂量联合组的细胞活力值分别为1.00±0.14,0.35±0.66,0.41±0.10,0.68±0.09,0.41±0.13,0.60±0.12,0.44±0.14和0.71±0.11,摄取谷氨酸量分别为(65.29±0.89),(34.96±13.27),(57.33±18.56),(52.34±13.07),(55.50±4.54),(52.94±12.30),(48.73±11.27)和(52.15±7.52)nmol·L^-1。与模型组相比,正常组和3个高剂量组的细胞活力均显著升高,差异均有统计学意义(均P<0.001);与低剂量联合组相比,3个高剂量组的细胞活力均显著升高,正常组、低剂量丹参多酚酸组和低剂量血栓通组的摄取谷氨酸量均明显升高,差异均有统计学意义(均P<0.05);与高剂量联合组相比,3个低剂量组的细胞活力均显著降低,差异均有统计学意义(均P<0.05)。结论低剂量丹参多酚酸组和低剂量血栓通组可以提高受损星形胶质细胞谷氨酸摄取能力,高剂量丹参多酚酸组、高剂量血栓通组和低、高剂量联合组对受损星形胶质细胞谷氨酸摄取能力无影响。

Effects of Salvia miltiorrhiza and Panax notoginseng on glutamate uptake in astrocytes of rats with oxygen glucose deprivation injury

Objective To investigate the effects of single and combined use of effective components of salvianolic acids and Xueshuantong on glutamate uptake in oxygen glucose deprivation/reoxygention(OGD/R)induced astrocytes.Methods Primary Wistar rat cerebral cortex astrocytes were cultured in vitro and divided into 8 groups:normal group,model group,low-dose and high-dose salvianolic acid groups,low-dose and high-dose Xueshuantong groups,and low-dose and high-dose combination groups.No drug was given in the normal and model groups.The low-dose and high-dose salvianolic acids groups were given 12.5 and 25.0 g·mL^-1 salvianolic acids,respectively.Low-dose and high-dose Xueshuantong groupss were given 3.125 and 6.25 g·mL^-1 Xueshuantong,respectively.The low-dose and high-dose combination groupss were given 12.5 g·mL^-1 salvianolic acid combined with 3.125 g·mL^-1 Xueshuantong and 25.0 g·mL^-1 salvianolic acid combined with 6.25 g·mL^-1 Xueshuantong,respectively.Normal group was given routine culture.The model group was treated with OGD 3 h/R 3 h.The administration group was treated with OGD 3 h/R 3 h after 2 days of administration.Cell activities and glutamate uptake contents were detected by enzyme labeling instrument.Results The cell activities of normal group,model group,low-dose and high-dose salvianolic acid groups,low-dose and high-dose Xueshuantong groups,and low-dose and high-dose combination groups were 1.00±0.14,0.35±0.66,0.41±0.10,0.68±0.09,0.41±0.13,0.60±0.12,0.44±0.14 and 0.71±0.11,the glutamate uptake contents were(65.29±0.89),(34.96±13.27),(57.33±18.56),(52.34±13.07),(55.50±4.54),(52.94±12.30),(48.73±11.27)and(52.15±7.52)nmol·L^-1.Compared with the model group,the cell activities of normal group and three high-dose groups were significantly increased(all P<0.001).Compared with the low-dose combination group,the cell activities of three high-dose groups were significantly increased,the glutamate uptake contents of normal group,low-dose salvianolic acid group,low-dose Xueshuantong group were significantly increased(all P<0.05).Compared with the high-dose combination group,low-dose groups were significantly decreased(all P<0.05).Conclusion The low-dose salvianolic acid and low-dose Xueshuantong groups could improve the glutamate uptake ability of the damaged astrocytes,while the high-dose salvianolic acid group and high-dose Xueshuantong group and low and low-dose and high-dose combination groups had no effect on the glutamate uptake ability of the damaged astrocytes.