| ITS2序列鉴定小茴香及其常见混伪品 |
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| 引用本文： | 刘义梅,罗焜,陈科力,陈士林. ITS2序列鉴定小茴香及其常见混伪品[J]. 环球中医药, 2011, 4(4): 260-263. DOI: 10.3969/j.issn.1674-1749.2011.04.006 |
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| 作者姓名： | 刘义梅 罗焜 陈科力 陈士林 |
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| 作者单位： | 1. 湖北中医药大学省部共建中药资源和中药复方教育部重点实验室,武汉,430065
2. 中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室 |
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| 基金项目： | 卫生部卫生行业科研专项 |
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| 摘 要： | 目的 探讨基于ITS2序列鉴定小茴香及其混伪品的新方法.方法 对小茴香4份样品的ITS2序列进行PCR扩增和测序,同时从GenBank上下载小茴香及其常见混伪品共6个物种42个样本.用MEGA4.1计算其种间、种内的K-2-P距离,并分析各物种间ITS2序列二级结构的差异,最后利用ITS2序列构建其系统发育树.结果 小...
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| 关 键 词： | 小茴香 ITS2序列 分子鉴定 |
Molecular identification ofFoeniculum vulgareand its adulterants by ITS2 sequence |
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| LIU Yi-mei,LUO Kun,CHENKe-li,et al.. Molecular identification ofFoeniculum vulgareand its adulterants by ITS2 sequence[J]. Global Chinese Medicine, 2011, 4(4): 260-263. DOI: 10.3969/j.issn.1674-1749.2011.04.006 |
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| Authors: | LIU Yi-mei LUO Kun CHENKe-li et al. |
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| Affiliation: | LIU Yi-mei,LUO Kun,CHENKe-li,et al.Hubei University ofChinese Medicine,Wuhan 430061,China |
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| Abstract: | Objective To explore the new method of discriminatingFoeniculum vulgarefrom its adulterants by using ITS2 sequence.Method DNA samples were extracted fromFoeniculum vulgare.ITS2 sequence was amplified by PCR and sequenced bidirectionally.Sequences were assembled by CodonCode Aligner.The intra-specific and inter-specific K-2P distances ofFoeniculum vulgareand its adulterants were calculated,and NJ tree was constructed by MEGA V4.1.ITS2 and secondary structure were predicted by the web tool established by Schu... |
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| Keywords: | Foeniculum vulgare ITS2 Molecular identification |
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