云芝子实体多糖化学结构的研究

目的　研究云芝子实体中多糖的化学结构。方法　应用柱色谱方法进行分离、纯化得一均一多糖组份B-1-3。通过完全水解、甲基化、一维和二维¹H和¹³CNMR阐明B-1-3的化学结构。结果　经完全水解证实B-1-3主要由Glc单糖残基组成,用HPLC方法测得其分子量为3.16×10⁵,通过对甲基化反应数据和¹H和¹³CNMR分析发现B-1-3为由β-D-1,4-Glc和β-D-1,3-Glc残基构成主链的葡聚糖,同时在β-D-1,3,6-Glc及β-D-1,4,6-Glc残基处产生分枝。经¹H和¹³CNMR分析表明B-1-3分子中存在β-D-Glc-1→3-β-D-Glc-β-D-Glc-1→4-β-D-Glc及β-D-Glc-1→4-β-D-Glc-β-D-Glc-1→4-β-D-Glc片段。结论　云芝子实体多糖B-1-3为由β-D-1,4-Glc,β-D-1,3-Glc和β-D-1,6-Glc残基构成主链的葡聚糖。

STUDIES ON CHEMICAL STRUCTURE OF POLYSACCHARIDE FROM FRUIT BODY OF CORIOLUS VERSICOLOR

AIM: To study the structure of polysaccharides from Coriolus versicolor. METHODS: By chromatography a homogeneous polysaccharide B-1-3 was obtained. The structure of B-1-3 was elucidated using the method of total acid hydrolysis, methylation, and 1D and 2D NMR. RESULTS: B-1-3 was found to be mainly composed of Glc by total acid hydrolysis. Its molecular weight was estimated to be 3.16 x 10(5) by HPLC method. Methylation analysis and 1H and 13CNMR indicated the main chain of B-1-3 consisted of beta-D-1,4-Glc and beta-D-1,3-Glc, and branch chains were situated, beta-D-1,3,6-Glc and beta-D-1,4,6-Glc. 1H and 13CNMR also showed that beta-D-Glc-1-->3-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc and beta-D-Glc-1-->4-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc fragments existed in B-1-3. CONCLUSION: B-1-3 is a glucan which main chain was composed of beta-D-1,4-Glc and beta-D-1,3-Glc beta-D-1,6-Glc.